. The cytoplasm of the plant cell. Plant cells and tissues; Protoplasm. Guilliermond - Atkinson 130 — Cytoplasm always appear in much smaller numbers than when obtained by vital staining or after fixation. These bodies, therefore, are gen- erally the result of flocculation of a substance found normally in a colloidal solution in the vacuolar sap. This flocculation occurs in the presence of vital dyes or fixatives. P. A. Dangeard kept for this substance the name metachromatin which we had pro- posed to designate the substance constituting the metachromatic corpuscles (volutin of ARTHim Meyer).
. The cytoplasm of the plant cell. Plant cells and tissues; Protoplasm. Guilliermond - Atkinson 130 — Cytoplasm always appear in much smaller numbers than when obtained by vital staining or after fixation. These bodies, therefore, are gen- erally the result of flocculation of a substance found normally in a colloidal solution in the vacuolar sap. This flocculation occurs in the presence of vital dyes or fixatives. P. A. Dangeard kept for this substance the name metachromatin which we had pro- posed to designate the substance constituting the metachromatic corpuscles (volutin of ARTHim Meyer). P. A. Dangeard had the idea of trying the effect of vital stains, among others cresyl blue, on a very large number of cells of the most varied plant groups. In every one he found that there existed a colloidal substance dispersed in the vacuolar sap possessing a strong capacity for taking up vital stains which precipitate it in the form of corpuscles showing Brownian movement. These he identified with the metachromatin of fungi. Thus he arrived at the conclusion that all vacuoles enclose metachromatin, a specific sub- stance of vacuoles, and he states that vital staining thus constitutes a property of the vacuoles which is both general and characteristic. Studying the origin of vacuoles in most varied plant cells by staining them with the vital dye, cresyl blue, Dangeard demon- strated that vacuoles exist in all embryonic cells but are very different in ap- pearance from ordinary vacuoles. They are seen here as numerous minute elements composed of a very concentrated solution of metachro- matin in a semi-fluid state. By their forms, as well as by their dimensions, they are decidedly reminiscent of the chondriosomes. It is these elements, swelling by imbibition and coalescing, which finally become the large vacuoles characteristic of mature cells. We shall not dwell on this matter here, but will study it later in more detail. Our research immediately afterward, confirmed, in p
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