. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 100 Figure 3. Effects of protein kinase C (PKC) modulators on larval settle- ment and metamorphosis. (A) Induction of settlement and metamorphosis by dibutyrate (PDBLI): Cupilella metatrochophore larvae, less than 1 day after release, were exposed to the PKC activator phorhol PDBLI added to the seawater at concentrations ranging from 1 to 25 jiM, and settlement and metamorphosis was noted after 1 h. Controls received 50 /al 95% ethanol. Bars represent the percent settlement and metamorphosis for each concentration (mea
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 100 Figure 3. Effects of protein kinase C (PKC) modulators on larval settle- ment and metamorphosis. (A) Induction of settlement and metamorphosis by dibutyrate (PDBLI): Cupilella metatrochophore larvae, less than 1 day after release, were exposed to the PKC activator phorhol PDBLI added to the seawater at concentrations ranging from 1 to 25 jiM, and settlement and metamorphosis was noted after 1 h. Controls received 50 /al 95% ethanol. Bars represent the percent settlement and metamorphosis for each concentration (mean ± SEM. n = 6. representing (SO larvae/concentration). Asterisks indi- cate significant differences (P 25) between control and exper- imental values at each concentration tested by one-way ANOVA with a Bonterroni correction for multiple comparisons where a = (Bl Inhibi- tion of juvenile hormone (JH) Ill-induced settlement and metamorphosis by H-7. Cn/iitclUi metatrochophore larvae, less than 1 day after release, were pre-exposed lor 3 h to concentrations of H-7. a PKC inhibitor, in seawater ranging from 1 to 100 /xA/. before being exposed to JH III (20 /jjV/) lor I h. Controls (0 '7) received 100 Ml 45<7r ethanol followed by JH III. Bars represent the percent settlement and metamorphosis for each concentration after the 4-h time period (mean ± SEM, n = 6. representing 60 larvae/ concentration). Asterisks same as in (A). Activation of u protein kimiae C-like enzyme in Capitella larvae b\ JH-active chemicals Although PKC is considered to be a ubiquitous enzyme present throughout the animal kingdom and has been found in marine sponges (Muller ct a/.. 19H7) and Dictwsti'Iimu discoideum (Jimenez et a/., 1989; Luderus et al., 1989). its presence in polychaetes has so far not been documented. The presence of a PKC-like enzyme in Capitella larvae was therefore investigated, as was also the ability of JH-active compounds to activate this enzyme. In these investigations,
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
