. dan bai zhi zu xue cong xu lie dao gong neng. botany. 158 èç½è´¨ç»å-¦ æ§æèèç½çå离ï¼èåºå®å梯度è¶æ¡æ´æ¹ä¾¿ï¼å¸¸è§ä½¿ç¨æ´å®¹æï¼Lopez, 1999b; Pat- ton etal., 1999b)ãææè¿äºåæ°ç»åèµ·æ¥ä½¿è¶ä¸è¶ä¹é´çæç¹ä½ç½®ç¸å ³æ§å¾å¥½ï¼ä¸ç®¡ æ¯å¨IEF (ç-çµèç¦ï¼è¿æ¯åå-è´¨éå离ï¼åå¼é½å°äº1% (Fishmann, 1999; Lopez and Patton, 1997)ãå¾æ¾ç¤ºçæ¯6åä¸åçè¡æ¸ 2-DEè¶å¨åä¸åºåçæ¾å¤§ç §çï¼ å¨ç¾ç æ ·åä¸-å¯çå°åºç°äºå¨å¯¹ç §æ ·åä¸-没æçæ°æç¹ãé¤éè¶ä¸è¶ä¹é´ç模å¼ï¼pat- tern) éç°æ§é常好ï¼å¦åæ¯ä¸å¯è½è¿è¡è¿ç§æ¯è¾çã (a) (b) å¯¹ç §ç» 1. å¾è¡æ¸ èç½2-DEåè¶å¾åé æ¯åºåæ¾å¤§å¾ (a-c)å¯¹ç §æ ·åãï¼d-f)ç¾ç æ ·åãåè¶å离ååææ¡ä»¶è§Lopez an
. dan bai zhi zu xue cong xu lie dao gong neng. botany. 158 èç½è´¨ç»å-¦ æ§æèèç½çå离ï¼èåºå®å梯度è¶æ¡æ´æ¹ä¾¿ï¼å¸¸è§ä½¿ç¨æ´å®¹æï¼Lopez, 1999b; Pat- ton etal., 1999b)ãææè¿äºåæ°ç»åèµ·æ¥ä½¿è¶ä¸è¶ä¹é´çæç¹ä½ç½®ç¸å ³æ§å¾å¥½ï¼ä¸ç®¡ æ¯å¨IEF (ç-çµèç¦ï¼è¿æ¯åå-è´¨éå离ï¼åå¼é½å°äº1% (Fishmann, 1999; Lopez and Patton, 1997)ãå¾æ¾ç¤ºçæ¯6åä¸åçè¡æ¸ 2-DEè¶å¨åä¸åºåçæ¾å¤§ç §çï¼ å¨ç¾ç æ ·åä¸-å¯çå°åºç°äºå¨å¯¹ç §æ ·åä¸-没æçæ°æç¹ãé¤éè¶ä¸è¶ä¹é´ç模å¼ï¼pat- tern) éç°æ§é常好ï¼å¦åæ¯ä¸å¯è½è¿è¡è¿ç§æ¯è¾çã (a) (b) å¯¹ç §ç» 1. å¾è¡æ¸ èç½2-DEåè¶å¾åé æ¯åºåæ¾å¤§å¾ (a-c)å¯¹ç §æ ·åãï¼d-f)ç¾ç æ ·åãåè¶å离ååææ¡ä»¶è§Lopez and Patton, 1997,ä¸åä¹å¤å¨ äºä½¿ç¨äº&010«86 2-0£åæ软件çï¼GSI)ãç®-头ææå¤ä¸ºå¨ç¾ç æ ·åä¸-å·®å¼è¡¨è¾¾çèç½è´¨ã (èS. Rasheed, UCLAæ å ï¼ å¯éååé¨æ¶éææ¯ å°½ç®¡2-DEæ¯ç®åå离èç½è´¨æ··åç©çå ·æé«å辨ççææ¯ï¼å®ä»ç¶ä¸è½å辨å¨ä»» ä¸ç»èä¸-ææ表达çèç½è´¨ï¼ã10ä¸ä¸ªï¼ï¼å¤§å¤æ°ç 究è ç¨å¯éæé¢åå¸æ¶éææ¯æ¥å¯» æ±è§£å³è¿ä¸é®é¢çåæ³ï¼å¦äº²åè²è°±ï¼æº¶æ¶²ä¸-åé¨æ¶éæå¨2-DEåè¿è¡äºç»èåé¨æ¶ é (Harry et al. , 2000 ï¼ Lopez, 1999a; Lopez et al. , 2000a; Quadroni and James, 1999)ãå·²ç»å¼ååºçå¦ä¸ç§åé¨æ¶éæ段æ¯å¨ä¸ç»´ç-çµèç¦å离ä¸-使ç¨çªèå´çåºå® åpH梯度è¶ï¼1ã2个pHåä½ï¼ï¼Harry efaZ.ï¼ 2000; APB, BioRad, Proteome Sys- tems, GSI) o æ¾åææ¯ ç®å2-DE
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