. DNA ji RNA ji ben shi yan ji shu. botany. 第äºåç« åºç¨ç-循ç¯-åè±æ°§æ³ è¿è¡DNAæµåº Barton E. Slatko . 1.å¼ è¨ è®¸å¤å®éªå®¤ä¸-DNAæµåºå·²ç»å¾å¿«æ为æ¥å¸¸å·¥ä½çä¸é¨åï¼å¤ç§ä¸åçæ°çå éè½½ ä½ãæµåºç-ç¥åææ¯å·²ç»åå±èµ·æ¥ï¼ä»¥ä¾¿æ´ææå°æµå®åç§DNA模æ¿ã尽管ç®åå ³æ³¨ çç¦ç¹ä¸»è¦å¨èªå¨DNAæµåºæ¹é¢ï¼ä½å¨èªå¨åæµåºæ¹æ³å¹¿æ³åºç¨ä¹åï¼ä»ç¶é¡»ç¨å ¶ä» æ¹æ³ãæè¿å¨äººå·¥æµåºæ¹é¢åå¢å äºä¸ç§æ°çæ¹æ³ï¼ç§°ä½ç-循ç¯æµåºã ç-循ç¯æµåºæ¹æ³çåºç¡æ¯Sangerç-çåè±æ°§æ ¸è·é ¸é¾ç»æ-¢æ³[1]ãå¨è¿ä¸ååºä¸-ä¸ ä¸ªåéçå¼ç©DNAåå-ä¸äºè¡¥çä¸æ®µDNAåé¾è¿è¡å¤æ§ï¼å°è¿ä¸å¼ç©æ¨¡æ¿å¤åç©å èç-DNAèåé ¶ä¸èµ·ä¿æ¸©ï¼æç¨çèç-é ¶æ¯Thermococcus litoralisçå¤åæ ¸
. DNA ji RNA ji ben shi yan ji shu. botany. 第äºåç« åºç¨ç-循ç¯-åè±æ°§æ³ è¿è¡DNAæµåº Barton E. Slatko . 1.å¼ è¨ è®¸å¤å®éªå®¤ä¸-DNAæµåºå·²ç»å¾å¿«æ为æ¥å¸¸å·¥ä½çä¸é¨åï¼å¤ç§ä¸åçæ°çå éè½½ ä½ãæµåºç-ç¥åææ¯å·²ç»åå±èµ·æ¥ï¼ä»¥ä¾¿æ´ææå°æµå®åç§DNA模æ¿ã尽管ç®åå ³æ³¨ çç¦ç¹ä¸»è¦å¨èªå¨DNAæµåºæ¹é¢ï¼ä½å¨èªå¨åæµåºæ¹æ³å¹¿æ³åºç¨ä¹åï¼ä»ç¶é¡»ç¨å ¶ä» æ¹æ³ãæè¿å¨äººå·¥æµåºæ¹é¢åå¢å äºä¸ç§æ°çæ¹æ³ï¼ç§°ä½ç-循ç¯æµåºã ç-循ç¯æµåºæ¹æ³çåºç¡æ¯Sangerç-çåè±æ°§æ ¸è·é ¸é¾ç»æ-¢æ³[1]ãå¨è¿ä¸ååºä¸-ä¸ ä¸ªåéçå¼ç©DNAåå-ä¸äºè¡¥çä¸æ®µDNAåé¾è¿è¡å¤æ§ï¼å°è¿ä¸å¼ç©æ¨¡æ¿å¤åç©å èç-DNAèåé ¶ä¸èµ·ä¿æ¸©ï¼æç¨çèç-é ¶æ¯Thermococcus litoralisçå¤åæ ¸é ¸é ¶æ´»æ§ ç¼ºé·çDNAèåé ¶ãVentR⢠(exo" ) DNAèåé ¶[2'3]ææ¯77ierm"5 ag"^mV"s (Taq) èåé ¶[4~7]ï¼æ-¤å¤å å ¥ä¸ç£·é ¸è±æ°§æ ¸è·ï¼dNTPs)åä¸ç£·é ¸åè±æ°§æ ¸è·ï¼ddNTPs)ãå ç§åå¼çååºæ··åç©ï¼ï¼æ¯ä¸ååºæ··åç©ä¸-å«æåç§dNTPsååç§ddNTPsä¸-çä¸ç§ï¼ ç»ä¿æ¸©äº§çåå¥ä¸åé¿åº¦çç段ï¼æ¯å¥ç»æ-¢å¨ä¸ç¹å®æ ¸è·é ¸æ®åºä½ç½®ãå è¿éå¼ç©ã VentRâ¢DNAèåé ¶ãdNTPsåddNTPs,ä»å°éç模æ¿åå-ç»éå¤åæ§å¤æ§åé¾å»¶ä¼¸ ååºï¼å¯è·å¾ååºäº§ç©ç线æ§æ©å¢åè¾å¼ºçæµåºä¿¡å·ï¼å¾)ãå¨æ¯ä¸å¾ªç¯ä¸-ï¼ååº æ¸©åº¦åé«å°951C使åé¾æ¨¡æ¿æåé¾DNA模æ¿çäºçº§ç»æåæ§ï¼éä½å°551C以使模æ¿ä¸ å¼ç©å¤æ§ï¼ç¶åå°
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Keywords: ., bookcollectionbiodiversity, bookcontributorli, booksubjectbotany
