. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 92 MAURICE SUSSMAN typical fruits. Control of the number of myxamoebae delivered was achieved by - appropriate dilution of the original cell suspensions. The population density of the cells on the agar was determined by the volume of the drop delivered. RESULTS A. The growth curve of D. discoideum on complete and restricted media Spores, taken from a 4—6 day stock plate, were suspended in salt solution and spread on glucose peptone agar with cc. of a 48-hour broth culture of A. aero- genes. The initial spore count per pl
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 92 MAURICE SUSSMAN typical fruits. Control of the number of myxamoebae delivered was achieved by - appropriate dilution of the original cell suspensions. The population density of the cells on the agar was determined by the volume of the drop delivered. RESULTS A. The growth curve of D. discoideum on complete and restricted media Spores, taken from a 4—6 day stock plate, were suspended in salt solution and spread on glucose peptone agar with cc. of a 48-hour broth culture of A. aero- genes. The initial spore count per plate was 1 X 10'\ At intervals, duplicate plates were harvested using a finger and 10 cc. distilled water delivered in two batches. Control experiments using known numbers of cells indicated a recovery in excess of 95% of the cells on the plates. Replicate counts with the hemocy- tometer were made of each harvest. Figure 1 shows the results of two experiments (upper curve). A technical difficulty precluded the use of this system for studying the aggrega- •z. • . 10 TIME IN HRS. FIGURE 1. Growth curves of D. discoideum on complete (I) and restricted (II) media. The arrows indicate the times at which cell samples were taken for tests of aggregative Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Marine Biological Laboratory (Woods Hole, Mass. ); Marine Biological Laboratory (Woods Hole, Mass. ). Annual report 1907/08-1952; Lillie, Frank Rattray, 1870-1947; Moore, Carl Richard, 1892-; Redfield, Alfred Clarence, 1890-1983. Woods Hole, Mass. : Marine Biological Laboratory
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
