. Adventures in radioisotope research;. Radioactive tracers; Radiobiology. 276 ADVENTURES IN RADIOISOTOPE RESEARCH We determined the blood phosphorus of the laying hen denoted as I, the result being seen from Table 3. The blood phosphorus of another lien is discussed on p. 274. Table 3. P-CONTENT OF HeNS BlOOD iu plasma mgm "/(, mgin % ill oorpuscles iu blood Phospliatide P Inorganic P Total acid soluble P Rest (Protein) P . .. In the blood of non-laying hens(J> after 24 hours fasting an average phosphatide P content of mgm% was found the total plasma P amounting to
. Adventures in radioisotope research;. Radioactive tracers; Radiobiology. 276 ADVENTURES IN RADIOISOTOPE RESEARCH We determined the blood phosphorus of the laying hen denoted as I, the result being seen from Table 3. The blood phosphorus of another lien is discussed on p. 274. Table 3. P-CONTENT OF HeNS BlOOD iu plasma mgm "/(, mgin % ill oorpuscles iu blood Phospliatide P Inorganic P Total acid soluble P Rest (Protein) P . .. In the blood of non-laying hens(J> after 24 hours fasting an average phosphatide P content of mgm% was found the total plasma P amounting to 13 mgm%, the plasma inorganic P to mgm%. EXPERIMENTAL METHODS The yolk wus dried by adding ice cold acetoii, the dry yolk was carefully pulverised and the powder obtained shaken for 15 min with 150 ce. ether, the last mentioned procedure being repeated four times using fresh ether. The ether was than carefully evaporated, the residue taken up with dry ether, the latter removed by evaporation, this time in a Kjeldahl flask, and the residue ashed. The phosphatides of plasma, corpuscles and total blood were extracted b_\- an ether-alcohol mixture after Bloor. The extract was several times carefully evapo- lated to dryness and taken up with ether or petrol ether. The residue of the first extraction was treated with trichloracetic acid (10 cc. of 10% solution for each cc. of blood) and from the filtrate obtained the inorganic P precipitated as ammo- nium magnesium phosphate; the esters present in the filtrate were hydrolised and the phosphate produced by the hydrolysis of the esters precipitated as ammo- nium magnesium phosphate. Though the extraction and the neutrahsation of the acid solution were both carried out at —9°, some of the inorganic phosphorus present may be due to decomposition of the esters and we therefore gave in the table only the total acid soluble phosphorus present in the corpuscles which includes both the inorganic and the ester phosphorus. The liver was
Size: 1921px × 1300px
Photo credit: © Library Book Collection / Alamy / Afripics
License: Licensed
Model Released: No
Keywords: ., bookcentury1900, bookcollectionameri, bookcollectionbiodiversity
