. The cytoplasm of the plant cell. Plant cells and tissues; Protoplasm. GuilHermond - Atkinson — 194 — Cytoplasm very earliest investigations, the hypothesis that the Golgi apparatus might well correspond to a vacuolar system analogous to that of plant cells. Moreover, we had shown that, by means of Regaud's method, the young filamentous and reticulate vacuoles may be seen as a network of colorless canaliculi within the grey-tinted cyto- plasm and present absolutely the aspect of the canaliculi of Holm- gren. That led us to think that the apparatus of Golgi and that of Holmgren might perhaps b
. The cytoplasm of the plant cell. Plant cells and tissues; Protoplasm. GuilHermond - Atkinson — 194 — Cytoplasm very earliest investigations, the hypothesis that the Golgi apparatus might well correspond to a vacuolar system analogous to that of plant cells. Moreover, we had shown that, by means of Regaud's method, the young filamentous and reticulate vacuoles may be seen as a network of colorless canaliculi within the grey-tinted cyto- plasm and present absolutely the aspect of the canaliculi of Holm- gren. That led us to think that the apparatus of Golgi and that of Holmgren might perhaps be one and the same formation, corre- sponding to certain phases of the vacuolar system analogous to that of plant cells. A little later, with Mangenot, we tried to verify this hypothesis in the meristem cells of the barley root (Fig. 129) which, as we have seen, contain small filamentous vacuoles, very characteristic and easy to bring out by vital staining with neutral red. Treating these cells by the method recommended by Bensley for detection of the canaliculi of Holmgren, we succeeded in obtaining images very comparable to those of an apparatus of Holmgren formed of colorless canaliculi, appearing as if punched out against the grey cytoplasmic background. These, in diiferentiat- ing cells, swell and coalesce and, in the mature cell, are transformed into large vacuoles. Moreover, in treating the same root with the sil- ver impregnation methods which Golgi employed to bring out his re- ticular apparatus, we obtained in the cells of the meristem a network like that of Golgi, corresponding ex- actly to the apparatus of Holmgren, obtained by the methods of Bensley, and to the filamentous and reticulate phases of the vacu- olar system, as they appear after vital staining with neutral red. These observations which seemed to verify our hypothesis, were later confirmed in animal cytology by the work of A. CORTI, then of Parat and of his collaborators. CoRTi proved, in fact, that
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Keywords: ., bookcentury1900, bookcollection, booksubjectplantcellsandtissues