. Biological stains; a handbook on the nature and uses of the dyes employed in the biological laboratory. Stains and staining (Microscopy); Stains and Staining; Dyes. Phenyl Methane Dyes 145 ably less subject to fading. Slides have been exposed to direct sunlight for a few weeks and have still retained the green color. This dye is therefore to be recommended for such use as it has all the advantages of light green without the disadvantages. Con- sidering the comparatively short period of time since this dye was first introduced to the microscopist, it is now employed in a sur- prisingly large
. Biological stains; a handbook on the nature and uses of the dyes employed in the biological laboratory. Stains and staining (Microscopy); Stains and Staining; Dyes. Phenyl Methane Dyes 145 ably less subject to fading. Slides have been exposed to direct sunlight for a few weeks and have still retained the green color. This dye is therefore to be recommended for such use as it has all the advantages of light green without the disadvantages. Con- sidering the comparatively short period of time since this dye was first introduced to the microscopist, it is now employed in a sur- prisingly large number of procedures, among which might be men- tioned the following as illustrating two widely different types of .7- o H O .5- £.3- UJ o O o o. 380 I I I I I I 440 500 560 620 WAVELENGTH TTI^A I J 680 Fig. 19. Spectral curves of three phenyl methane dyes: 1. Methyl green. 2. Light green SFY. 3. Fast green FCF. methods: the quadruple staining technic of Johansen (1939), for plant histology, in which it is combined with safranin, methyl violet, and orange G; its use as a bacterial stain by Maneval, (1941), who recommends this and other acid dyes ( acid fuch- sin, aniline blue , and orseilline) as preferable to the conven- tional basic dyes because of the better diflFerentiation afforded and the less tendency for slime and debris to take the Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Conn, H. J. (Harold Joel), 1886-1975. Geneva, N. Y. , Biotech Publications
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