. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. HATCHING ENVELOPE ASSEMBLY IN LOW SODIUM 89. Figure 14 A-F. A series of phase micrographs of living eggs showing the altered formation of the HE in both Tracliypenueiis Mitiilis and Sicyimia ingentis in reduced Na+ seawater. (A) A T. similis egg with jelly coat (arrow) in ChCI-substituted seawater at 4-5 min post spawning. (B) A crenated 5. ingentis egg in ChCl-substituted seawater at 12 min. (C) A 5 ingemis egg has rounded up in ChCI-substituted seawater by 35 min showing a typical morphology for both species. (D) A phase m
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. HATCHING ENVELOPE ASSEMBLY IN LOW SODIUM 89. Figure 14 A-F. A series of phase micrographs of living eggs showing the altered formation of the HE in both Tracliypenueiis Mitiilis and Sicyimia ingentis in reduced Na+ seawater. (A) A T. similis egg with jelly coat (arrow) in ChCI-substituted seawater at 4-5 min post spawning. (B) A crenated 5. ingentis egg in ChCl-substituted seawater at 12 min. (C) A 5 ingemis egg has rounded up in ChCI-substituted seawater by 35 min showing a typical morphology for both species. (D) A phase micrograph at 20 min of a S. ingenlis egg spawned into Tris-substituted seawater showing the prolonged irregular morphology of eggs in this media. (E) The collapsed HE (arrow) around the S. ingenlis egg in ChCI-substituted seawater at 65 min. (F) Collapsed HEs (arrow) around T vmilis eggs in ChCI-substituted seawater at 65 mm. Bar = 100 jim for all phase micrographs. a wave-like fashion. Two populations of granules also have been identified in the T. similis egg (Fig. 8) and appear similar to the dense and ring vesicles in the 5. ingentis egg (Pillai and Clark, 1988: Lynn et al., 1991). The dense vesicles migrate to the cortex and line up just beneath the plasmalemma by 30 min post spawning. This population of vesicles is the first to undergo exocytosis. Subsequently, the ring vesicles contained in cisternal elements migrate to the cortical cytoplasm and undergo exocytosis at a slightly later time. The morphology of the HE formation around S. in- gentis eggs has been previously reported by Pillai and Clark (1988). The formation of the HE around T. similis eggs is similar. It progresses from a fibrous, non-laminar struc- ture (Fig. 9) to a well defined bilayered structure (Fig. 11). By 60 min post-spawning, the HE consists of a dense outer stratum covering a thicker more flocculent inner layer (Fig. 11) similar to that observed in S. ingentis (Fig. 13). A space between the oolemm
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
