. Bulletin. Natural history; Natural history. September, 1955 Forsberg: Fusarium Disease of Gladiolus 471 and probably more biological races of F. oxysporum f. tracheiphilum Sny. & Hans, on the basis of pathogenicity on varieties of soybeans and cowpeas. Studies on the relation of culture types and pathogenicity of the 40 isolates of gladiolus Fusariutii listed in table 1 were undertaken by the writer with the hope of finding a possible relation between colony type, disease form, and degree of virulence. Methods of determining virulence are de- scribed under "Pathogenicity
. Bulletin. Natural history; Natural history. September, 1955 Forsberg: Fusarium Disease of Gladiolus 471 and probably more biological races of F. oxysporum f. tracheiphilum Sny. & Hans, on the basis of pathogenicity on varieties of soybeans and cowpeas. Studies on the relation of culture types and pathogenicity of the 40 isolates of gladiolus Fusariutii listed in table 1 were undertaken by the writer with the hope of finding a possible relation between colony type, disease form, and degree of virulence. Methods of determining virulence are de- scribed under "Pathogenicity ; Culture Types of the Gladiolus Fusariuw.—Original isolations from dis- eased gladiolus corms were made on po- tato dextrose agar. Growth forms of the original cultures were not recorded for all isolates. However, 23 of the isolates were classified as belonging to the raised, intermediate, or appressed types on the basis of their aerial mycelium, table 10. Later, all isolates were grown on the me- dium found by Wellman (1942) to give more distinctive reactions between cul- tural variants. This medium, referred to as Wellman's agar in the present investiga- tion, has the following composition: pro- teose peptone grams, dihydrogen pc tassium phosphate gram, magnesium sulfate gram, maltose grams, fer- rous sulfate gram, agar grams, water 1, 49-31 Fig. 13.—Raised, appressed, and intermediate growth forms of vascular isolates of Fusarium grown on Wellman's agar. The extreme raised and extreme appressed forms are shown in cul- tures 45-73 and 49-15, respectively. Cultures are 20 days Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Illinois. Natural History Survey Division. Urbana, State of Illinois, Dept. of Registration and Education, Natural History Survey
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