. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. CELL DIVISION. CELL MOTILITY, AND DEVELOPMENT 233. Figure 1. Swimming pluteus larva of Lytechinus variegatus (sea urchin). The stereo pair is arranged for cross-eved viewing. Each view wax computed from a stack ofca. 100 optical sections which were acquired in DIC microscopy at 30 sections/s, then digitally processed to remove out-of-focus hu:c digital contrast enhancement (3). We also described a method for very rapidly obtaining serial optical sections, repeatedly in time lapse, using video microscopy combined with synchro
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. CELL DIVISION. CELL MOTILITY, AND DEVELOPMENT 233. Figure 1. Swimming pluteus larva of Lytechinus variegatus (sea urchin). The stereo pair is arranged for cross-eved viewing. Each view wax computed from a stack ofca. 100 optical sections which were acquired in DIC microscopy at 30 sections/s, then digitally processed to remove out-of-focus hu:c digital contrast enhancement (3). We also described a method for very rapidly obtaining serial optical sections, repeatedly in time lapse, using video microscopy combined with synchronous computerized control of the objective and condenser fine-focus controls (4). We now demonstrate, at these General Scientific Meetings, through-focal and three-dimensionally reconstructed stereo- scopic images of a free-swimming, pluteus-stage sea urchin em- bryo and the mitotic spindle fibers within the two-cell stage of a dividing ctenophore egg. The former, generated from serial optical sections in DIC microscopy, shows details of the external morphology, including the presence of a scoop on the mouth, and internal organs of the pluteus, with unexpected clarity (Fig. 1). Stereo views of sequential slices, generated from limited sets of serial optical sections, bring out further details of the cellular organization, including the regular pavement-like arrangement of the circumoral epithelium and the shape of the mesenchyme cells that were masked by the overlapping complex structure in the whole embryo. The spindle of the dividing ctenophore egg. reconstructed from serial optical sections in polarization microscopy, shows the 3- D organization of the birefringent chromosomal spindle fibers (kinetochore microtubule bundles) in rocking stereoscopic pro- jection. The fibers appear unusually well differentiated from the background array of microtubules, which was presumably aided by the averaging algorithm in the computational reconstruction that we used to generate the 3-D pr
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
