. Applied immunology : the practical application of sera and bacterins prophylactically, diagnostically, and therapeutically. i:j H 15 16 17 18 19 20 Fig. 29.—Opsonizer or thermostat. This incubator is equipped with an automaticheating device and permits of the ready manipulation of pipettes introduced throughthe side port-holes. THE OPSONIC INDEX 237 ? manner equal quantities of control serum, bacterialsuspension and washed leucocytes are taken up andmixed in pipette C (Fig. 28, III and IV). Thetips are sealed in a flame and the two pipettes incu-bated for a few minutes in a thermostat or ops
. Applied immunology : the practical application of sera and bacterins prophylactically, diagnostically, and therapeutically. i:j H 15 16 17 18 19 20 Fig. 29.—Opsonizer or thermostat. This incubator is equipped with an automaticheating device and permits of the ready manipulation of pipettes introduced throughthe side port-holes. THE OPSONIC INDEX 237 ? manner equal quantities of control serum, bacterialsuspension and washed leucocytes are taken up andmixed in pipette C (Fig. 28, III and IV). Thetips are sealed in a flame and the two pipettes incu-bated for a few minutes in a thermostat or opsonizerat 37° C. (Fig. 29). Care should be taken to agitate the contents every five min-utes during incubation,otherwise the corpuscleswill gravitate and a per-fect admixture of serum,bacteria and phagocyteswill not be obtained. Atthe conclusion usually offifteen minutes, smears aremade of the contents of thepipettes on cover-slips bythe usual method or glassslides, employing Kuhn-hardts spreader (Figs. 30and 31). After drying,the smears are fixed in absolute alcohol and stainedwith freshly filtered carbolthionin or
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Keywords: ., bookcentury1900, bookdecade1910, bookpublisherphila, bookyear1916
