. Cytological technique; the principles underlying routine methods. Histology -- Technique; Cytology -- Technique. INTRODUCTION TO FIXATION 27 they often look remarkably lifelike. Embedding in paraffin, how- ever, usually causes serious distortion. Ideally a fixative would leave the volume'of a piece of tissue volume as percentage of original volume 100 200 300 400. chromium trioxide osmium tetroxide mercuric chloride formaldehyde potassium dichromate acetic acid Fig. 5. Diagram showing the changes in vohime undergone by gelatine-albumin gel when acted upon for 18 hours by the selected primary
. Cytological technique; the principles underlying routine methods. Histology -- Technique; Cytology -- Technique. INTRODUCTION TO FIXATION 27 they often look remarkably lifelike. Embedding in paraffin, how- ever, usually causes serious distortion. Ideally a fixative would leave the volume'of a piece of tissue volume as percentage of original volume 100 200 300 400. chromium trioxide osmium tetroxide mercuric chloride formaldehyde potassium dichromate acetic acid Fig. 5. Diagram showing the changes in vohime undergone by gelatine-albumin gel when acted upon for 18 hours by the selected primary fixatives. The latter were used at their standard ^ and of its constituent cells unaltered, and resistant to alteration by fluids in which it was subsequently placed. Many fixatives shrink tissues; some leave them scarcely changed in volume, while others swell them, but in any case the tissue is liable to subsequent shrinkage. The results of shrinkage and swelling lend themselves more. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Baker, John Randal, 1900-. London, Methuen; New York, Wiley
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