. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 344 J. A. SCHWARZ AND V. M. WEIS. Figure 2. Transmission electron micrographs of iramunogold-labeled sections from tentacles of aposym- biotic anemones. Gold spheres, \isible as black dots, indicate the presence of s\m?2. lA) Epidermal cells with nematocysts (n). (B) Enlargement of the boxed section of A. with gold spheres labeling \esicles located near nematocysts. (C) Gastrodermal cells adjacent to the gastric cavity (go. (D) Enlargement of the boxed section of C. showing gold spheres within vesicles in the gastrodermal ce
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 344 J. A. SCHWARZ AND V. M. WEIS. Figure 2. Transmission electron micrographs of iramunogold-labeled sections from tentacles of aposym- biotic anemones. Gold spheres, \isible as black dots, indicate the presence of s\m?2. lA) Epidermal cells with nematocysts (n). (B) Enlargement of the boxed section of A. with gold spheres labeling \esicles located near nematocysts. (C) Gastrodermal cells adjacent to the gastric cavity (go. (D) Enlargement of the boxed section of C. showing gold spheres within vesicles in the gastrodermal cells. Scale bars = 2 (A. C) and 1 ^m (B. D). label was associated with the multiple membranes that enclose the dinoflagellate sxmbiont within the host cell (Fig. 3A, B). Gold spheres were diffusely arranged within these membranes, not clearly associated with any single membrane layer. To confirm that this labeling was specific tn :he membranous lasers, we quantified the staining rela \ .• to areas outside the membranes. The membranous contained an average ot gold spheres ± <SD), n =- I1), while equivalent areas outside the membranous layers contained an average of ± , n = 19). Symbiodinium witliin host cells. In addition to the svm32 labeling \\ithin the membranes that surround the symbionts. there was a significant amount of labeling within the symbionts themselves (Fig. 4). Specifically, gold spheres were located within the accumulation body, a poorly described organelle that is believed to function in the endocytic pathways of dinoflagellates. The density of labeling within the accumulation bodies was highly variable: some contained only a few gold spheres, while others contained hundreds (average = ± [SD] gold spheres/jiAm2, n = IS).. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble
Size: 1514px × 1650px
Photo credit: © Library Book Collection / Alamy / Afripics
License: Licensed
Model Released: No
Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
