. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 90 K A. BROWNE AND R. K. ZIMMER Table 1 Mean (±SEM) concentrations of disodium fluorescein (fjLg/ml) and gl\c\/-g/vcv/-L-arginine (GGR; nM) in waters sampled from among the shell substrata of lan-al collectors Time (h) since collector was placed in the field Compound 1 2 24 36 48 60 72 Disodium fiuorescein 0. 18 ± GGR — ± 58 ± 13 ± ± 59 ± 16 ± ± 45 ± 18 Time = 0 is defined as the point at which collectors were first submerged in field habitats and for which neither fluor
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 90 K A. BROWNE AND R. K. ZIMMER Table 1 Mean (±SEM) concentrations of disodium fluorescein (fjLg/ml) and gl\c\/-g/vcv/-L-arginine (GGR; nM) in waters sampled from among the shell substrata of lan-al collectors Time (h) since collector was placed in the field Compound 1 2 24 36 48 60 72 Disodium fiuorescein 0. 18 ± GGR — ± 58 ± 13 ± ± 59 ± 16 ± ± 45 ± 18 Time = 0 is defined as the point at which collectors were first submerged in field habitats and for which neither fluorescein nor GGR had yet been released from polyacrylamide gels. Separate experiments were performed for fluorescein and GGR. Initial concentrations of the compounds in acrylamide gels were 1 mg/ml (fluorescein) and 2 x 10~4 M (GGR). Seawater samples of fluorescein and GGR were collected at 12-h and 24-h intervals, respectively. nificantly more barnacle larvae than did either ASW or GGH (Fig. 3). Because GGR and GGH are basic peptides with nearly identical molecular weights and chemical functional- ities, settlement induction cannot be attributed to stimulation by organic compounds in general but is due. specifically, to the arginine moiety at the carboxy terminus of the peptide. These results show that even weakly swimming iarvae like barnacle cyprids (mean speed: mm s '; 24) can 25- 20- I 15- 10-. ASW GGH GGR Figure 3. The numbers (mean ± SEM; n = 8) of newly metamor- phosed barnacle juveniles (Balanus amphitrite) on clean oyster shells, provided as settlement substrata, were counted for chemical-releasing collectors (CRCs) with either no organic enrichment (ASW, control) or with 2 x 10~4 M GGR or GGH. Significantly more barnacle juveniles were found in response to GGR than to the control or GGH (P < one-way ANOVA followed by multiple, paired comparisons using Bon- ferroni's correction; 27). All CRCs were oriented with their openings facing upward wit
Size: 1986px × 1258px
Photo credit: © Library Book Collection / Alamy / Afripics
License: Licensed
Model Released: No
Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
