. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. Fill maze to 5cm depth I Place stimulus animal in left or right arm behind gate (position X) Leave for 1 minute I Place test male at start position (*) of maze i Allow 30 minutes before recording position onses: ( + ) Animal is in arm with stimulus (-) Animal is in opposite arm to stimulus (NC) No choice, in bottom on "Y" B Figure 3. (A) Schematic diagram of Y-maze for the investigation of pheromonal attraction of male Harmothoe imbricata to various stimuli. Dashed lines represent removable partitions. Depth of sea
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. Fill maze to 5cm depth I Place stimulus animal in left or right arm behind gate (position X) Leave for 1 minute I Place test male at start position (*) of maze i Allow 30 minutes before recording position onses: ( + ) Animal is in arm with stimulus (-) Animal is in opposite arm to stimulus (NC) No choice, in bottom on "Y" B Figure 3. (A) Schematic diagram of Y-maze for the investigation of pheromonal attraction of male Harmothoe imbricata to various stimuli. Dashed lines represent removable partitions. Depth of seawater in Y-maze: 5 cm. (B) Summary flow diagram of the experimental protocol. negative if it was in the opposite arm, and a no choice if it was in the base of the Y. During an experimental run, all potential stimulus individuals were alternated between each arm in consecutive experiments and cross-tested with all test males. Tests performed. All tests were performed within 2 weeks of collection. To assess for any directional bias ("handed- ness") and for chance levels of attraction in the maze, two sets of experiments were performed in which males were placed in the Y-maze with no stimulus animal. In the first experiment, nine old males were each run three times in the Y-maze and the position of the animal was recorded after 10 min. From observations of the males, it was suspected that this time period was not sufficient for the test animals to complete exploratory behavior before settling. A further six old males were tested without a stimulus six times each, and their position was recorded after 30 min. All subsequent tests were run for 30 min. Old males were tested against each of the following: old mature females (two sets) and old females carrying fertilized oocytes. New males were tested against each of the following: new mature females, new females maintained in short days, and old males. All statis- tical significance was assessed using \2 analyses. Results. T
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
