. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. SPECTRAL SENSITIVITY OF PANULIRUS 273 M C V CO 4) O) o -2 -3- dark-adapted. yellow-adapted o' /; / / ,6: A blue-adapted 350 400 450 500 550 Wavelength (nm) 600 650 FIGURE 2. Spectral sensitivity of a dark adapted eye, derived from intracellular recording (filled circles, upper curve). The maximum sensitivity is at about 510 nm. When spectral sensitivity is measured in the presence of colored backgrounds, the shape of the spectral sensitivity function is unaltered (lower 2 curves). having a maximum sensitivity in the ultravio
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. SPECTRAL SENSITIVITY OF PANULIRUS 273 M C V CO 4) O) o -2 -3- dark-adapted. yellow-adapted o' /; / / ,6: A blue-adapted 350 400 450 500 550 Wavelength (nm) 600 650 FIGURE 2. Spectral sensitivity of a dark adapted eye, derived from intracellular recording (filled circles, upper curve). The maximum sensitivity is at about 510 nm. When spectral sensitivity is measured in the presence of colored backgrounds, the shape of the spectral sensitivity function is unaltered (lower 2 curves). having a maximum sensitivity in the ultraviolet (Xmax near 370 nm). The former seems the more abundant, as it dominates the sensitivity of the dark-adapted eye. Microspectrophotometry of the main rhabdom demonstrated the presence of one pigment with a maximal absorption at 510 nm. Over 30 scans of randomly selected areas of main rhabdoms revealed only the 510 nm pigment. An example is shown in Figure 4. In order to obtain a difference spectrum, the pigment was bleached by orange light at pH 9, a treatment known to cause the photobleaching of crustacean rhodopsin (Bruno et al, 1977). The metarhodopsin was not studied. DISCUSSION Pamdirus rhodopsin in digitonin solution has Xmax at 504 nm (Fernandez, 1965), which is in reasonable agreement with both our electrophysiological and microspec- trophotometric measurements. The data do not allow us to conclude that the 6 nm difference is real. Fernandez found the Xmax of Panulirus metarhodopsin at 495 Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Marine Biological Laboratory (Woods Hole, Mass. ); Marine Biological Laboratory (Woods Hole, Mass. ). Annual report 1907/08-1952; Lillie, Frank Rattray, 1870-1947; Moore, Carl Richard, 1892-; Redfield, Alfred Clarence, 1890-1983. Woods Hole, Mass. : Marine Biol
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