Physiology and biochemistry in modern medicine . curred will become greatly aggravated if venous stasis has been produced in drawing the specimen for analysis. But the chief reason whythis method has not been extensively employed, as pointed ui by VanSlyke, is the technical difficulty of making the necessary analysis. It is most satisfactory to colled xenons blood after a period (one hourat least) of muscular rest (so that there is no excess of C02) and withoutvenous stasis, and to centrifuge without permitting any considerable lossof carbonic acid. The latter precaution is necessary because t
Physiology and biochemistry in modern medicine . curred will become greatly aggravated if venous stasis has been produced in drawing the specimen for analysis. But the chief reason whythis method has not been extensively employed, as pointed ui by VanSlyke, is the technical difficulty of making the necessary analysis. It is most satisfactory to colled xenons blood after a period (one hourat least) of muscular rest (so that there is no excess of C02) and withoutvenous stasis, and to centrifuge without permitting any considerable lossof carbonic acid. The latter precaution is necessary because there is amigration of acid radicles, e. g., HC1, from plasma into corpuscles whenthe C02 of the former is increased, and in the reverse direction when theC02 is decreased. If the C02 in the blood Avere not the same during cen-trifuging as it is in the body, the separate plasma would not contain thesame amount of alkali—i. e., its reserve alkalinity would be theoretically, therefore, centrifuging should be performed in. Fig. 10.—Diagram of apparatus for saturating blood or plasma with expired air. The glassbeads in the bottle condense excess of moisture. The separating funnel, as soon as it has beenfilled with expired air, should be closed by a stopper and the stopcock turned off. It is thenrotated so that the blood forms a film on its walls. an atmosphere containing the same partial pressure of C02 as exists inthe body (i. e., the alveolar air) (see page 344), this has been found im-practicable for general use, and is unnecessary if loss of C02 from thespecimen of blood is prevented by allowing it to flow into the syringevery slowly (without any suction). It is mixed in the syringe withpowdered (neutral) potassium oxalate (enough to make a 1 per centsolution with the blood), and immediately delivered into a centrifugetube under paraffin oil, which by floating on its surface serves to diminishfree diffusion of C02 to the outside air (even though such oils di
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