Examination of the urine; a manual for students and practitioners . distilled water Mix and cool. Stain, when cold, for thirty seconds. Great care shouldbe taken not to overstain with the brown color, as the purple bacteriamay take on a brownish hue if too much brown is used. The gonococciappear brown, likewise the pus-cells and the epithelia. The pseudo-gonococci and other Gram-positive germs appear purple. 334 EXAMINATION OF THE URINE Instead of the Bismark brown a weak aqueous solution of fuchsin or ofeosin may be used, staining the gonococci red, while the Gram-positivegerms stain pur
Examination of the urine; a manual for students and practitioners . distilled water Mix and cool. Stain, when cold, for thirty seconds. Great care shouldbe taken not to overstain with the brown color, as the purple bacteriamay take on a brownish hue if too much brown is used. The gonococciappear brown, likewise the pus-cells and the epithelia. The pseudo-gonococci and other Gram-positive germs appear purple. 334 EXAMINATION OF THE URINE Instead of the Bismark brown a weak aqueous solution of fuchsin or ofeosin may be used, staining the gonococci red, while the Gram-positivegerms stain purple. With the red stains there is rather more danger ofoverstaining the contrast color than with the brown, but in either casegreat care should be taken to guard against this error. V. The film is now washed for a few seconds in water and is driedand mounted in balsam,sary for the accurate study of gonococci. An oil-immersion lens y^-inch focus is neces- There is a diplococcus closely resembling the gonococcus,and decolorizing with Grams method, which can only be. Fig. 83.—Pus from gonorrhea, showing gonococci (Jakob). distinguished by cultural methods. In men this diplo-coccus oocurs in but 4 to 5 per cent, of chronic urethralinfections. Hence in 96 per cent, of cases Grams stainis accurate in revealing the gonococcus in the male. When diplococci of the characteristic kidney shape occurwithin the bodies of pus-cells and do not stain purple withthis method, one may be quite satisfied, if the patient isof the male sex, that the gonococcus of Neisser is women, however, no method short of a culture gives MICRO-ORGANISMS 335 positive assurance that we are dealing with the true gono-coccus, as the female genitals often harbor diplococci nega-tive to Gram and resembling gonococci in other respects,which prove to be non-gonorrheal germs on culture. It is in chronic gonorrhea, writh scarcely any dischargeexcepting a morning drop and a few shreds, that gonococciare looked f
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