. The Biological bulletin. Biology; Zoology; Marine biology. 108 DANIEL P. CHENEY except cells of the granulocytic series were absent in both living and stained preparations. Morphometric and enumeration data are presented in Table I. The average hematocrits were ± (N = 29) for caudal vessel samples and ± (N = 9) for ventral aorta samples obtained prior to blood volume determinations. The nearly 3% difference between the two mean hemato- crits was not significant (analysis of variance test; F = , 1, 36), and probably was a reflection of differences in sampling. Sam


. The Biological bulletin. Biology; Zoology; Marine biology. 108 DANIEL P. CHENEY except cells of the granulocytic series were absent in both living and stained preparations. Morphometric and enumeration data are presented in Table I. The average hematocrits were ± (N = 29) for caudal vessel samples and ± (N = 9) for ventral aorta samples obtained prior to blood volume determinations. The nearly 3% difference between the two mean hemato- crits was not significant (analysis of variance test; F = , 1, 36), and probably was a reflection of differences in sampling. Sample hematocrits from ventral aorta cannulations, used for total blood volume determinations, presented a lower average ( ±, N = 7) than presample hematocrits. This was a result of the decrease in sample size and not of blood loss. There was also a tendency for hematocrits (caudal vessel samples) to decrease with increasing fish weights. For a wide weight range, this may be correlated with activity or oxygen consumption. 201. HOURS IN CIRCULATION Figure 2. Whole blood ^^Cr activity; plasma ^^Cr activity ratios in three Tilapia versus the time of circulation. The ^^Cr uptake curve (Fig. 1) is nearly linear with activity maximizing at 75 to 90 minutes after the addition of °^Cr, although uptake is still rapid at 75 minutes. The dose-dependent effects or possible toxic levels of the °^Cr were not tested. Plasma or unlabeled ^^Cr activity levels in Tilapia decreased to near background levels 4 to 19 hours after ^^Cr infusion in the circulatory system. Initially a large quantity of the radioactivity present in the injection medium does not appear in the red cells. There is a steady decline of plasma activity levels after injection, however, indicating that plasma ^^Cr is being rapidly removed from circulation and stored or excreted or both. In all fish samples (serial and single samples from 8 fish) the whole blood activity:plasma activity ratios in the injection medium


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