. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 19%' . • .--• •*•' v- ,' •" 20 * FIGURE 18. Incorporation of 14C-cholesterol into the cytoplasm of the prothoracic glands. Grains are spread throughout the cytoplasm at the beginning of incorporation; scale 10 p. FIGURE 19. Clusters of labelled substances appear in the peripheral region of the cyto- plasm, and represent the secretory granules ; scale 25 /t- FIGURE 20. Migration of labelled substances from the prothoracic glands (left) to the hemolymph ; scale 10 /*. ecdysone was more than that of a-ecdysone. The radioac
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 19%' . • .--• •*•' v- ,' •" 20 * FIGURE 18. Incorporation of 14C-cholesterol into the cytoplasm of the prothoracic glands. Grains are spread throughout the cytoplasm at the beginning of incorporation; scale 10 p. FIGURE 19. Clusters of labelled substances appear in the peripheral region of the cyto- plasm, and represent the secretory granules ; scale 25 /t- FIGURE 20. Migration of labelled substances from the prothoracic glands (left) to the hemolymph ; scale 10 /*. ecdysone was more than that of a-ecdysone. The radioactivity of ecdysones was not detected in either the anterior part with the prothoracic glands or the posterior part without the prothoracic glands in the diapausing prepupae. Similar results were found in the isolated abdomens of Bombyx mori (Moriyama et al., 1970; Nakanishi et al., 1972), Mamestra brassicae (Gersch and Stiirzebecher, 1971), and Musca domestica (Studinger and Willig, 1975). These experiments showed that ecdysone biosynthesis occurred not only in the prothoracic glands but also in the peripheral tissues without prothoracic glands. However, most ecdysones were bio- synthesized in the prothoracic glands. Ecdysone biosynthesis in the prothoracic glands in vitro. To examine the re- sults in the former section, more direct experiments were performed in vitro. At first, the prothoracic glands of the post-diapausing stage were cultured in the syn- thetic medium CSM-2F for five days with the addition of labelled cholesterol at the onset of cultivation. The same treatment was also performed in the prothoracic glands of the diapausing stage as a control. After cultivation, ecdysone biosynthesis. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Marine Biological Laboratory (Woods Hole, Mass. );
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
