The Journal of biological chemistry . Fig. 1. Bence-Jones protein. Recrystallized three times. acetic acid, as described above, could not be crystallized. Whensmaller quantities of acetic acid were used in the precipitation,some crystalUzation occurred. 25 cc. of urine were precipitatedwith cc. of sodium sulfate after adding 3 cc. of glacial aceticacid. The pH was roughly 5. The precipitate was removed bycentrifugation and dissolved in a small quantity of water with theaid of a little sodium hydroxide. The solution was slightly acidi-fied, seeded, and allowed to stand at room temperature.
The Journal of biological chemistry . Fig. 1. Bence-Jones protein. Recrystallized three times. acetic acid, as described above, could not be crystallized. Whensmaller quantities of acetic acid were used in the precipitation,some crystalUzation occurred. 25 cc. of urine were precipitatedwith cc. of sodium sulfate after adding 3 cc. of glacial aceticacid. The pH was roughly 5. The precipitate was removed bycentrifugation and dissolved in a small quantity of water with theaid of a little sodium hydroxide. The solution was slightly acidi-fied, seeded, and allowed to stand at room temperature. A smallquantity of crystals was obtained after several days. Experiments were made acidifying the urine with sulfuric acidinstead of acetic and precipitating with sodium sulfate. Good D. Wright Wilson 209 yields of ciystals were obtained quickly when from 5 to 10 dropsof 10 per cent sulfuric acid were added to 25 cc. of urine, the mostacid solution being neutral to methyl orange. When 15 dropswere added (pH ± 3) the yield was much
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