. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 194 REPORTS FROM THE MBL GENERAL SCIENTIFIC MEETINGS d. Kaltenbach, J., K. Hudock, M. Burger, G. Misevic, and W. J. Kuhns. 1996. Bio/. Bull. 191: 296-297. 7. Kornfeld, S., and R. Kornfeld. 1985. Anim. Rev. Biochem. 54: 631-664. 8. Kaushal, G., and A. Elbein. 1994. Methods En-ymol. 230: 316- 320. 9 Reck, F., M. Springer, H. Paulson, I. , M. Sarkar, and H. Schachter. 1994. Carhohyjr. Res. 259: 93-101 10. Humphreys, T., and E. Reinherz. 1994. Imiminol. Today 15: 316- 320. 11 Fernandez-Busquets, X., and M. Burger. 19
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 194 REPORTS FROM THE MBL GENERAL SCIENTIFIC MEETINGS d. Kaltenbach, J., K. Hudock, M. Burger, G. Misevic, and W. J. Kuhns. 1996. Bio/. Bull. 191: 296-297. 7. Kornfeld, S., and R. Kornfeld. 1985. Anim. Rev. Biochem. 54: 631-664. 8. Kaushal, G., and A. Elbein. 1994. Methods En-ymol. 230: 316- 320. 9 Reck, F., M. Springer, H. Paulson, I. , M. Sarkar, and H. Schachter. 1994. Carhohyjr. Res. 259: 93-101 10. Humphreys, T., and E. Reinherz. 1994. Imiminol. Today 15: 316- 320. 11 Fernandez-Busquets, X., and M. Burger. 1999. Microsc. Res. Tech. 44: 204-218. Reference: Bio/. Bull. 199: 194-195. (October 2000) A Ca+2-Independent Cytolytic System from the Blood of the Marine Snail, Busycon caiialiculum Peter B. Armstrong* and Rengasamy Asokan (Molecular and Cellular Biology, University of California, Davis. California 95616) The immune system is the ensemble of cell-based and humoral agents that protect the organism against parasites that have gained access to the internal milieu. One of the important immune activ- ities for a variety of animals is the outright lysis of foreign cells that contact the blood (1). Cytolytic systems are frequently ex- plored as if they were hemolytic systems, where the foreign cell that is destroyed is the mammalian erythrocyte. The red cell is convenient for the assay of cytolysis because its hemolysis is easily quantified by the release of hemoglobin into the bathing medium. Here we report the presence of a hemolytic system in the plasma of the marine snail. Busycon. Blood was obtained as follows. The foot of the adult whelk was wounded, and the blood flowing from the wound was collected and then immediately centrifuged to remove the cells. Hemocyanin was removed from the plasma by centrifugation ( RPM. 4 h). The hemocyanin-free plasma was dialyzed into Tris-buffered saline ( M NaCl, 50 m/WTris, pH ). Hemolysis was assayed with rabbit red cells as descr
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