. Pathogenic micro-organisms, including bacteria and Protozoa; a practical manual for students, physicians and health officers. Fig. 77.—One of the very characteristic forms of diphtheria bacilli from blood-serum cultures,showing clubbed ends and irregular stain. X 1100 diameters. Stain, methylene blue. Fig. 78.—Extremely long form of diphtheria bacillus. This culture has grown on artificialmedia for fifteen years and produces great amounts of toxin. X1100 diameters. Fig. 79 Fig. 80 ■>\ ^ -^^> ^ >^ . H \. FiQ, 79.—Diphtheria bacilli characteristic in shapes, but showing even staining.
. Pathogenic micro-organisms, including bacteria and Protozoa; a practical manual for students, physicians and health officers. Fig. 77.—One of the very characteristic forms of diphtheria bacilli from blood-serum cultures,showing clubbed ends and irregular stain. X 1100 diameters. Stain, methylene blue. Fig. 78.—Extremely long form of diphtheria bacillus. This culture has grown on artificialmedia for fifteen years and produces great amounts of toxin. X1100 diameters. Fig. 79 Fig. 80 ■>\ ^ -^^> ^ >^ . H \. FiQ, 79.—Diphtheria bacilli characteristic in shapes, but showing even staining. X1000diameters. Stain, methylene blue. Fig. 80.—Non-virulent diphtheria bacilli, showing stain with Neisser s solutions. This appear-ance was formerly supposed to be characteristic of virulent baciUi. Bodies of bacilU in smear,yellowish-brown; points, dark blue. to eighteen hours growth on serum, nearly all will show the blue bodies (), while with the pseudo type (Fig. 86, page 206), to be described hereafter,few will be seen. The solutions are as follows: No. 1. Alcohol (96 per cent.) 20 parts. Methylene blue (Grubler) 1 part. Distilled water !)50 parts. Acetic acid (glacial) oO parts. No. 2. Bismarck brown 1 part. Boiling distilled water oOO parts. The Neisser stain has been advocated in order to separate the viru-lent from the non-virulent l^acilli, without the deUiy of inoculating 198 PA THOGENIC MICRO-ORGA NISMS. animals; but in our hands, with a very large experience, neither theNeisser
Size: 1586px × 1575px
Photo credit: © Reading Room 2020 / Alamy / Afripics
License: Licensed
Model Released: No
Keywords: ., bookcentury1900, bookdecade1910, bookpublishernewyo, bookyear1910
