. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. A CYTOTOXIN FROM BLEPHARISMA 147 vores would eat Blepharisma, Actinosphaerium was tried. Not only did this heliozoan feed upon Blepharisma but it did so voraciously and individuals of the latter were not only engulfed but digested. Almost as soon as a suspension of Blepharisma was added some were caught in the extended axopodia of the heliozoan. Sometimes on struggling they succeeded in breaking loose, but more often they did not. Within a few minutes they were engulfed in the streaming protoplasm and enclosed in a vacuole w
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. A CYTOTOXIN FROM BLEPHARISMA 147 vores would eat Blepharisma, Actinosphaerium was tried. Not only did this heliozoan feed upon Blepharisma but it did so voraciously and individuals of the latter were not only engulfed but digested. Almost as soon as a suspension of Blepharisma was added some were caught in the extended axopodia of the heliozoan. Sometimes on struggling they succeeded in breaking loose, but more often they did not. Within a few minutes they were engulfed in the streaming protoplasm and enclosed in a vacuole which was drawn towards the body. After several hours some individuals of Actinosphaerium had as many as twelve deep red vacuoles. After several more hours they were surrounded with red fecal deposits. 5 r i k O Uj 13 PARAMECIUM &ALONE © / PAR. WITH I BL. Q I PAR. WITH 4 ALONE O / BL. WITH I PAR. C24 BL. WITH I PAR. 10 20 30 40 SO TIME IN HOURS AFTER INOCULATION FIGURE 1. Comparison of division-rates of Paramecium in the presence and absence of Blepharisma. Three sets of eight cultures each were used for these determinations. In addition another series in which two instead of four Blepharisma were studied and still another with eight Blepharisma. All the experiments indicated the same result. If one observes the food vacuoles within an Actinosphaerium, one will see that the contents decrease in size and become more intensely colored. As digestion pro- ceeds the vacuoles do not seem to change hue, always appearing red. Sometimes the fluid within the vacuole turns pink. However the protoplasm of Actinosphaerium never takes on a reddish color. Actinosphaerium fed on Blepharisma continues to grow and divide. Whether division would go on at the same rate as on other food could not be determined since division occurred in such an erratic manner. The latter is probably due to the fact that Actinosphaerium is multinucleate and may. Please note that these images are extracted f
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
