. Bulletin of the British Museum (Natural History). BIOLOGY OF EUGLYPHA ROTUNDA (PROTOZOA) 127. TIME IN DAYS Fig. I. Growth curves for E. rotunda in culture. The symbols represent tliree replicate cultures through which curves have been fitted by eye. The calculated doubling times are ? — ? i-g days ; 0 © x 1-4 days and —— x 1-85 days. FOOD Although the cultures in the laboratory were normally grown on a mixed bac- terial flora, it is noted that successful cultures can be maintained using a single bacterium as the sole food source. Agar plates, streaked with Klebsiella aerogenes (strain
. Bulletin of the British Museum (Natural History). BIOLOGY OF EUGLYPHA ROTUNDA (PROTOZOA) 127. TIME IN DAYS Fig. I. Growth curves for E. rotunda in culture. The symbols represent tliree replicate cultures through which curves have been fitted by eye. The calculated doubling times are ? — ? i-g days ; 0 © x 1-4 days and —— x 1-85 days. FOOD Although the cultures in the laboratory were normally grown on a mixed bac- terial flora, it is noted that successful cultures can be maintained using a single bacterium as the sole food source. Agar plates, streaked with Klebsiella aerogenes (strain NCIB 8017) and covered with the Euglypha culture medium, were incubated overnight at 25°C to establish the bacteria. The plates were then inoculated with washed Euglypha and incubated in the dark at 20 °C. Sub-cultures were made at weekly intervals and under these conditions the Euglypha appeared to grow and reproduce Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original British Museum (Natural History). London : BM(NH)
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