. A text-book of bacteriology; a practical treatise for students and practitioners of medicine. Bacteriology. RABIES 653 the passage of the virus through twenty-one to thirty rabbits, in this way, will reduce its incubation time to seven or eight days. Babes claims to obtain a virus fixe more rapidly by passing the virus alter- nately through rabbits and guinea-pigs. For pm-poses of inoculation, virus is prepared by emulsifying in sterile salt solution pieces of the medulla or cerebellum of animals dead of. Fig. 153.—Method op Drying Spinal Cobd of or Attenuation. Rabbit tor Purposes a previou
. A text-book of bacteriology; a practical treatise for students and practitioners of medicine. Bacteriology. RABIES 653 the passage of the virus through twenty-one to thirty rabbits, in this way, will reduce its incubation time to seven or eight days. Babes claims to obtain a virus fixe more rapidly by passing the virus alter- nately through rabbits and guinea-pigs. For pm-poses of inoculation, virus is prepared by emulsifying in sterile salt solution pieces of the medulla or cerebellum of animals dead of. Fig. 153.—Method op Drying Spinal Cobd of or Attenuation. Rabbit tor Purposes a previous inoculation. The brain tissue which is not emulsified may be preserved under sterile glycerin in a dark and cool place for furthemse. II. Rabbits are inoculated with virus fixe by intracranial injection. A small incision is made in the shaved scalp in the median line, and the skin is retracted. With a small trephine or a round chisel, an opening is made in the skull in the angle between the coronary and sagittal su- tures. Through this opening about to of the virus fixe is in- jected, either directly into the brain substance or simply imder the dura. As soon as a rabbit so inoculated has died it is autopsied. The animal before dissection should be washed in a disinfectant solution —lysol or carbolic acid. The skin is then removed and the animal, lying on its ventral surface, is fastened to a dissecting board. The spinal canal is then laid open with a pair of curved scissors and the spinal cord carefully removed. This is accomplished by cutting across the cord in the liunbar region, and lifting this with a forceps while the nerve roots are divided from below upward. The cord is suspended by a sterile thread within a large bottle into the bottom of which pieces of potassium hydrate have been placed. The bottle is then set away in a dark room or closet, the temperature of. Please note that these images are extracted from scanned page images that may have been di
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