. Bacteriological methods in food and drug laboratories, with an introduction to micro-analytical methods. Bacteriology; Food; Drug adulteration. 134 BACTERIOLOGICAL METHODS. Fig. 43.—Tubercle ba- cillus slant culture on glyc- erin-agar, several months old.—{Stilt, after Curtis.) is not conclusive, the other'two inocu- lated guinea-pigs should be kept 2 weeks longer then dissected and examined like the first. Occasionally there is abscess formation at the point of inoculation but this need not necessarily interfere with the tubercular development in the glands and in the deeper tissues. It is
. Bacteriological methods in food and drug laboratories, with an introduction to micro-analytical methods. Bacteriology; Food; Drug adulteration. 134 BACTERIOLOGICAL METHODS. Fig. 43.—Tubercle ba- cillus slant culture on glyc- erin-agar, several months old.—{Stilt, after Curtis.) is not conclusive, the other'two inocu- lated guinea-pigs should be kept 2 weeks longer then dissected and examined like the first. Occasionally there is abscess formation at the point of inoculation but this need not necessarily interfere with the tubercular development in the glands and in the deeper tissues. It is frequently possible to isolate the bacillus of tuberculosis (from sputum, glandular tissues, meat pulp, centrifugal- ized sediments of milk, cream, etc.) by special manipulation and the use of special culture media. The following method is suggested. Spread two or three drops of the material (concentrate, sediment, crushed, suspected tuberculous meat ex- tract, etc.) evenly over the surface of two or three glass slips and place the smear preparations in the drying oven at 100° C. for 15 min., however, not before the ma- terial on the slips is well dried at the room temperature. Tubercle bacilli are quite resistant to dry heat and will withstand the temperature of 100° C. for from 30 min. to I hr. The exposurb to that tem- perature for 15 min. will kill most of the bacteria associated with the tubercle germs and will in fact kill some of these. At the end of 15 min. take the glass slips from the drying oven and by means of a small sterile spatula or scalpel, scrape the dried suspected material over the surface. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Schneider, Albert, 1863-1928. Philadelphia, P. Blakiston's son & co
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Keywords: ., bo, bookcentury1900, bookdecade1910, booksubjectfood, bookyear1915
