. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. COLL1SELLA AUSTRODIGITALIS SP. NOV. 195 Electro phorcsis The digestive glands of individual snails were removed and homogenized in 1 ml polethylene BEEM capsules using the roughened ends of glass rods as pestles. The capsules were centrifuged at 4500 X g for 10 min and the super- natant absorbed onto 5x6 mm pieces of Whatman 3MM filter paper for insertion into starch gels. Gel composition, electrophoresis, and the leucine aminopeptidase (Lap) staining method have been described (Murphy, 1976). Phosphoglucose isomerase (Pgi)
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. COLL1SELLA AUSTRODIGITALIS SP. NOV. 195 Electro phorcsis The digestive glands of individual snails were removed and homogenized in 1 ml polethylene BEEM capsules using the roughened ends of glass rods as pestles. The capsules were centrifuged at 4500 X g for 10 min and the super- natant absorbed onto 5x6 mm pieces of Whatman 3MM filter paper for insertion into starch gels. Gel composition, electrophoresis, and the leucine aminopeptidase (Lap) staining method have been described (Murphy, 1976). Phosphoglucose isomerase (Pgi) alloenzymes were visualized by staining for 5 to 10 min in a solution of 2 mg phenazine methosulfate, 7 mg NADP, 15 mg MTT, 20 mg fructose-6-phosphate, 30 units of glucose-6-phosphate dehydrogenase, and 160 mg MgCU-6H2O in 70 ml M Tris-HCl buffer. pH' Horse ferritin (Sigma) was used as a marker, and individuals of known genotypes served as standardizations of mobility variants between samples. RESULTS Lap polymorphisms The zymograms reveal one major zone of Lap activity, and, assuming a correspondence between bands and alleles, a total of 10 codominant alleles were found at the locus coding for this zone. The Lap phenotypes of homozygotes are double bands about 4 mm apart, and those of heterozygotes are four bands, or three where the fast band of the slower allele has the same mobility as the slow band of the faster allele (Fig. 2). Allele abbreviations follow Ayala, Powell, Tracey, Mourao, and Perez-Salas (1972}. The most common allele Origin 0. ©I04/ I08/ I04/ I08/ 100, /I f~\c\ / \r\r\ /\/-\ s\ /1 i o / u/ 108 '108 7I04 '112 XI08 North. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Marine Biological Laboratory (Woods Hole, Mass. ); Marine Biological Laboratory (Woods Hole, Mass. ). Annual repo
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
