. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. ELECTROLYTES AND ISLET METABOLISM 417 OXYGEN UPTAKE m>j* 02 PER >jg PROTEIN PER HOUR 1 1 WIDTH OF TISSUE SEAL (mm) FIGURE 1. Effect of cartesian diver tissue seal width on the respiration of islet slices sus- pended in M NaCl. All of the points on the graph in which the tissue seal width was mm. or less represent an average of 19 or more individual determinations; the other points repre- sent the average of 5 to 13 individual determinations. used, the reproducibility was no
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. ELECTROLYTES AND ISLET METABOLISM 417 OXYGEN UPTAKE m>j* 02 PER >jg PROTEIN PER HOUR 1 1 WIDTH OF TISSUE SEAL (mm) FIGURE 1. Effect of cartesian diver tissue seal width on the respiration of islet slices sus- pended in M NaCl. All of the points on the graph in which the tissue seal width was mm. or less represent an average of 19 or more individual determinations; the other points repre- sent the average of 5 to 13 individual determinations. used, the reproducibility was not as good as that observed in the saline-phosphate mixture. A possible explanation of this is shown in Figure 1. The volume of the tissue seal varied considerably from experiment to experiment and in Figure 1 the oxygen uptake of islet slices in M saline was plotted against the volume of tissue seal in the diver, , the volume of fluid medium surrounding the tissue slice. The metabolic activity of the islet slices in M saline decreased as the volume of the tissue seal increased. Moreover, this did not occur when phosphate was used; therefore, this greater metabolic variability in saline may be the result of variable dilution of the phosphate extracted from the tissue. Since phosphate addition stim- ulated the respiration of islet, one would expect that the oxygen uptake would be greater in the divers with small tissue seals. The oxygen uptake of the islet tissue slices, when expressed as m/A. of oxygen per TABLE II Effect of different proportions of Na+ to K+ on islet tissue respiration. All media contained M phosphate buffer pH + M NaCl or KCl. The proportions of Na+ to K+ were changed by varying the buffer and saline cations Ratio Na+/K+ in medium No. determinations Aver, m^l. O2/Mg. protein/hr. <7 100/0 * 16 15 * Saline-PO4 mixture as described in Table Please note that these images are extracted from scanned pa
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
