. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 476 M. A. YUI AND C. J. BAYNE in the first hour post-injection () by , followed by a period of slower clear- ance (Fig. 1). Clearance continued after 6 h (Fig. 2), and bacteria were not detected 4-8 days To further characterize the initial 90 min of clearance, four or five samples were removed from each of nine urchins previously injected with 105-107 bacteria ml"1 of CF. Clearance was exponential. Lines were fitted to the log-trans- formed data using linear regression by calculating independent slopes for


. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 476 M. A. YUI AND C. J. BAYNE in the first hour post-injection () by , followed by a period of slower clear- ance (Fig. 1). Clearance continued after 6 h (Fig. 2), and bacteria were not detected 4-8 days To further characterize the initial 90 min of clearance, four or five samples were removed from each of nine urchins previously injected with 105-107 bacteria ml"1 of CF. Clearance was exponential. Lines were fitted to the log-trans- formed data using linear regression by calculating independent slopes for each urchin and obtaining a mean and standard error for those slopes. The slopes are equivalent to the "Phagocytic Index" or K value as defined by Biozzi et al. (1953) for particle clearance kinetics in mammals, and Renwrantz and Mohr (1978) for particle clear- ance in a land snail, Helix. For the first 90 min of clearance, a K value of ± logic bacteria min"1 was obtained for the Gram negative (R2 = ). The slope was not strongly dose dependent (Yui, 1982). Because of individual differences, primary and secondary clearance rates were determined for the same individual urchins. Injection doses of 106-107 bacteria ml"1 of CF were selected for further experiments because, at those doses, bacteria were: 107H " , 106H DC LLJ \- o < CD 10-. 0 I I I I I 23456 TIME (hours) FIGURE 1. Primary clearance of the Gram negative and Gram positive marine bacteria, and Aero- monas salmonicida, sampled at 1, 3, and 6 hours post-injection (mean ± standard error). • Gram negative (n = 6), A Gram positive (n = 3), • Aeromonas salmonicida (n = 3).. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Marine Biological Laboratory (Woods Hole, Mass. ); Marine Biological Laboratory (Wo


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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology