. Ciba Foundation Symposium on the Regulation of Cell Metabolism. Cell metabolism. Role of TPN in Control of Glycolysis 235 Table I Phosphatase activities of liver mitochondria Mitochondria Pi liberated xpt. (200 ) Substrate (jxM/lO minutes) 1 Fresh G-6-P 1-36 2 Aged G-6-P 017 ATP 1-62 Each flask contained Tris-buffer pH 7 • 4; 3 [xm of substrate; 3 jjiM-MgCla in a total volume of 1 • 0 ml. P = inorganic phosphate. ably unable to oxidize pyruvate or to bring about oxidative phosphorylation (Siekevitz and Potter, 1955). Their G-6-Pase contamination is weak or absent, but they have a c
. Ciba Foundation Symposium on the Regulation of Cell Metabolism. Cell metabolism. Role of TPN in Control of Glycolysis 235 Table I Phosphatase activities of liver mitochondria Mitochondria Pi liberated xpt. (200 ) Substrate (jxM/lO minutes) 1 Fresh G-6-P 1-36 2 Aged G-6-P 017 ATP 1-62 Each flask contained Tris-buffer pH 7 • 4; 3 [xm of substrate; 3 jjiM-MgCla in a total volume of 1 • 0 ml. P = inorganic phosphate. ably unable to oxidize pyruvate or to bring about oxidative phosphorylation (Siekevitz and Potter, 1955). Their G-6-Pase contamination is weak or absent, but they have a considerable. 250 500 0 250 500 0 250 AGED LIVER MITOCHONDRIA () Fig. 2. Influence of variable amounts of aged mitochondria and of TPN on glycolysis. Each flask contained in a 1 • 6-ml. volume, 0*5 ml. brain-Sg, variable amounts of mitochondria as indicated, 7-5 [jtM glucose, 1 -5 (XM ATP, 0-5 [xm DPN, 60 (xm nicotinamide, 6 txM-MgClg, 10 [JIM potassium phosphate pH 7-4, 1-5 \jm TPN when indicated. 0-15 m-KCI to maintain isotonicity. ATPase activity (Table I). The addition of aged liver mito- chondria modifies the rate of glycolysis by the brain super- natant, as shown in Fig. 2, which also presents the effect of. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Ciba Foundation Symposium on the Regulation of Cell Metabolism (1958 : London, England); Wolstenholme, G. E. W. (Gordon Ethelbert Ward); O'Connor, Cecilia M. (Cecilia Mary), 1927-. Boston, Little, Brown
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