. Pathogenic micro-organisms, including bacteria and Protozoa; a practical manual for students, physicians and health officers. )ed at the end of twenty-four hours, riieseare round, but less coarsely granular, darker in color, and with a moresharply defined border than the comma bacillus. Liquefaction ofthe gelatin around these colonies progresses rapidly, and at the endof forty-eight hours is usually complete in |)lates where they are iiumer-. ^.: Spirilluin of Finkler and 1100 diameters. 456 PATHOGEXIC MICRO-ORGAXISMS. ous. In gelatin-stick cultures liquefaction progresses much morer


. Pathogenic micro-organisms, including bacteria and Protozoa; a practical manual for students, physicians and health officers. )ed at the end of twenty-four hours, riieseare round, but less coarsely granular, darker in color, and with a moresharply defined border than the comma bacillus. Liquefaction ofthe gelatin around these colonies progresses rapidly, and at the endof forty-eight hours is usually complete in |)lates where they are iiumer-. ^.: Spirilluin of Finkler and 1100 diameters. 456 PATHOGEXIC MICRO-ORGAXISMS. ous. In gelatin-stick cultures liquefaction progresses much morerapidly than in similar cultures of the cholera spirillum, and a stocking-shaped pouch of liquefied gelatin, already seen after forty-eight hours,is filled with a cloudy liquid. The liquefaction increases, and intwenty-four hours more reaches the sides of the tube in the upper partof the medium; by the end of the week the gelatin is usually completelyliquefied. Upon the surface of the liquefied medium a whitish filmis seen. Upon agar there is a somewhat more luxuriant growththan is seen with the cholera vibrio. Upon potato this spirillum growsat room temperature and produces a slimy, grayish-yellow, glisteninglayer which soon extends over the entire surface. The cholera spiril-lum does not grow at room temperature, and in the incubator producesa thin, brownish layer. The absence of agglutination with a suitabledilution of the serum of an


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