. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 208 K. A. SUPRENANT 23456. 205 116 97 66 45 36 1 Figure 8. Sedimentable luhulin was absent from homogenates prepared in a microtubule assembly buffer without hexylene glycol. Surf clam oocytes were homogenized in a buffer containing 100 K- PIPES, 4 mA/ EGTA. I MgSO4. I m\I DTT. PMSF, 10 ><g/ml leupeptin, and I m/U GTP, at either pH , or Homogenates were centnfuged at 2500g through a ICFr. sucrose cushion prepared at the appropriate pH. Proteins in the supernatant and pellet were separated b
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 208 K. A. SUPRENANT 23456. 205 116 97 66 45 36 1 Figure 8. Sedimentable luhulin was absent from homogenates prepared in a microtubule assembly buffer without hexylene glycol. Surf clam oocytes were homogenized in a buffer containing 100 K- PIPES, 4 mA/ EGTA. I MgSO4. I m\I DTT. PMSF, 10 ><g/ml leupeptin, and I m/U GTP, at either pH , or Homogenates were centnfuged at 2500g through a ICFr. sucrose cushion prepared at the appropriate pH. Proteins in the supernatant and pellet were separated by SDS-PAGE and electroblotted to nitrocellulose. Panel A is Western blot stained tor total protein with India ink (Hughes ct til. 1988). Panel A' is the corresponding immunoblot with the DM IA anti-a-tubulin antibody. Lanes I, 2. and 3 are the 2500g pellets from the pH , , and homogenates and Lanes 4, 5. and 6 are the corresponding si ivi nates at pH , , and Note the absence of pelletable luhulin in all three Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Marine Biological Laboratory (Woods Hole, Mass. ); Marine Biological Laboratory (Woods Hole, Mass. ). Annual report 1907/08-1952; Lillie, Frank Rattray, 1870-1947; Moore, Carl Richard, 1892-; Redfield, Alfred Clarence, 1890-1983. Woods Hole, Mass. : Marine Biological Laboratory
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