. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 234 REPORTS FROM THE MBL GENERAL SCIENTIFIC MEETINGS Reference: Biol. Hull. 201: 234. (October 21)01) Centrifuge Polarizing Microscope with Dual Specimen Chambers and Injection Ports Robert A. Kmulson (Marine Biological Laboratory, Woods Hole, Massachusetts), Slunva Inoue, and Makoto Goda] We reported earlier on a centrifuge polarizing microscope (CPM) that was designed for observing the weak birefringence of organelles and fine structures in living cells as they became stratified and ori- ented under centrifugal fields of u
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 234 REPORTS FROM THE MBL GENERAL SCIENTIFIC MEETINGS Reference: Biol. Hull. 201: 234. (October 21)01) Centrifuge Polarizing Microscope with Dual Specimen Chambers and Injection Ports Robert A. Kmulson (Marine Biological Laboratory, Woods Hole, Massachusetts), Slunva Inoue, and Makoto Goda] We reported earlier on a centrifuge polarizing microscope (CPM) that was designed for observing the weak birefringence of organelles and fine structures in living cells as they became stratified and ori- ented under centrifugal fields of up to times Faith's gravita- tional field. In this earlier model (1). one chamber A contained the specimen under observation, while the contents of the opposed second chamber B, which acted solely to balance the rotor, could not be viewed. We have now improved the CPM so that either chamber can be viewed and selected at the flick of a lever, within the duration ot a video frame. In the CPM, an electronic timing circuit synchronizes the firing of the light source laser precisely to the transit of the specimen under the microscope (freezing the image to less than specimen motion at up to rpm, regardless of the speed of the 16-cm diameter rotor). The timing circuit, in turn, is triggered by the signal from a photodiode that picks up the light originating from a stationary diode laser, and reflected by a small mirror (Ml) mounted on the spinning rotor near its axis. The complexity of the electronic timing circuit led us to keep the electronic circuit undis- turbed and instead to devise an optical system for switching between the display of the two chambers. To this end, we installed a second timing mirror (M2) on the rotor, exactly opposite the one for chamber A, but tilted up by a few degrees, rather than oriented parallel to the rotor axis as is Ml. In front of the photodiode we also placed a mounted pair of small mirrors on a "beam switcher" that
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
