. A text-book of bacteriology. Bacteriology. 138 PRODUCTS OF VITAL ACTIVITY. formed by oxidation of the alcohol, producing from it carbon diox- ide and water. The formation of acetic acid from alcohol is shown bj- the follow- ing formula : Ethyl alcohol ,.0H + 0, = CH^.COOH + Butyric acid is produced by a considerable number of bacteria, one of which, named Bacillus butyricus, has received the special at- tention of Prazmowski. This is strictly anaerobic. In solutions of starch, dextrin, sugar, or salts of lactic acid, when oxygen is ex- cluded it produces butyric acid in considerab


. A text-book of bacteriology. Bacteriology. 138 PRODUCTS OF VITAL ACTIVITY. formed by oxidation of the alcohol, producing from it carbon diox- ide and water. The formation of acetic acid from alcohol is shown bj- the follow- ing formula : Ethyl alcohol ,.0H + 0, = CH^.COOH + Butyric acid is produced by a considerable number of bacteria, one of which, named Bacillus butyricus, has received the special at- tention of Prazmowski. This is strictly anaerobic. In solutions of starch, dextrin, sugar, or salts of lactic acid, when oxygen is ex- cluded it produces butyric acid in considerable quantity, and at the same time carbon dioxide and hydrogen gas are set free. Duclaux gives the following formula of a solution containing lactate of lime in which the action of the butyric-acid ferment may be well studied : Water, Lactate of lime (pure). Phosphate of ammoiiia, Phosphate of potash,. Sulphate of magnesa, Sulphate of ammonia. 8 to 10 Utres. 235 grammes. " Fig. r?. This is introduced into a flask with two necks, such as is shown in Fig. 77. Having iilled the flask with the culture liquid, the bent neck is dipped into a porcelain dish containing the same. Heat is then applied both to flask and dish, and the liquid in each is kept in ebullition for half an hour. By this means the air is completely driven out of the flask. This is now allowed to cool, while the fluid in the shallow dish is kept hot, so that the liquid mounting from it into the flask shall be free from air. When the flask is full it is transferred to an incubating oven heated to 35° to 30° C, and the bent tube is immersed in a dish containing mercury. The Httle funnel. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Sternberg, George Miller, 1838-1915. New York, W. Wood and company


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