Essentials of bacteriology; being a concise and systematic introduction to the study of bacteria and allied microörganisms . rectedcentrally (Gen. 39 X 1500) (Novy, MacNeal, and Torrey). In the body fluids division occurs, first of the nucleus andthen of the protoplasm. Cultivation.—Novy and MacNeal have succeeded in culti-vating these protozoa on blood-agar, and multiplication goeson rapidly, so that rosettes are formed with the flagella ar-ranged around a common center. (See Figs. 102, 103, 104.) Trypanosoma Lewisi (Kent, 1878).—Found in rats byLewis; not fatal to them, though often equalin


Essentials of bacteriology; being a concise and systematic introduction to the study of bacteria and allied microörganisms . rectedcentrally (Gen. 39 X 1500) (Novy, MacNeal, and Torrey). In the body fluids division occurs, first of the nucleus andthen of the protoplasm. Cultivation.—Novy and MacNeal have succeeded in culti-vating these protozoa on blood-agar, and multiplication goeson rapidly, so that rosettes are formed with the flagella ar-ranged around a common center. (See Figs. 102, 103, 104.) Trypanosoma Lewisi (Kent, 1878).—Found in rats byLewis; not fatal to them, though often equaling the red cor-puscles in number. It was one of the first of this group to 2o6 ESSENTIALS OF BACTEKIOLOGY be described. The infection continues for two months with-out producing any illness, and the animal is then immune. Injection of infected rat blood into healthy rat causes thelatter to become infected. The injection of serum from an immune rat will preventthe disease in normal rats. Cultivated best at 20° C. and is very resistant to rat is probably infected by the bite of a flea or louse.(See Fig. 105.). Fig. 104.—Pure culture of trypanosomes of mosquitos—Crithidiafasciculata. Elongated crithidia from same preparation as preceding(Novy, MacNeal, and Torrey). Trypanosoma Brucei (Plimmer and Bradford, 1894) causes nagana, or tsetse-fly disease, a disease affecting horses,cattle, and dogs in certain regions of South Africa. ThetrjTpanosome of Bruce is less motUe than that of Lewis. Ithas been cultivated at 25° C, and is less resistant to laboratory animals subject to infection. The rat dies inten days. In the natural infection Bruce discovered that the tsetse-fly transmitted the disease, but that it did so by first bitingsome animal whose blood contained the trjrpanosome. The PROTOZOA 207 blood of infected animals contains the organism, and can, ifinjected, produce the disease without the agency of the far the tsetse-fly alone is responsible


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