. The actinomycetes. Actinomycetales. 52 THE ACTINOMYCETES, Vol. II. Figtjke 14. Orskov's motile Nocardia (Reproduced by courtesy of N. M. McClung] broth cultures, a thick, hard, orange to brownish surface pellicle is formed. Habitat: Soil. 41. Nocardia petroleophila Hirsch and Engel, 1950 (Hirsch, P. and Engel, H. Ber. deut. bot. Gesell. 69: 441-454, 1956). Morphology: Grows slowly, but abun- dantly on all mineral media, faster in a petroleum atmosphere. It grows on certain organic media, but does not produce any aerial mycelium. Substrate mycelium breaks up readily into rods; "involutio
. The actinomycetes. Actinomycetales. 52 THE ACTINOMYCETES, Vol. II. Figtjke 14. Orskov's motile Nocardia (Reproduced by courtesy of N. M. McClung] broth cultures, a thick, hard, orange to brownish surface pellicle is formed. Habitat: Soil. 41. Nocardia petroleophila Hirsch and Engel, 1950 (Hirsch, P. and Engel, H. Ber. deut. bot. Gesell. 69: 441-454, 1956). Morphology: Grows slowly, but abun- dantly on all mineral media, faster in a petroleum atmosphere. It grows on certain organic media, but does not produce any aerial mycelium. Substrate mycelium breaks up readily into rods; "involution cells" are formed abundantly. Mycelial threads are long, monopodially branched, to ju in diameter, and contain "metachromatic granules," readily stained with aqueous methylene blue. Aerial hyphae have the same diameter as the substrate hyphae, little branched. Aerial mycelium wets with difficulty. Xo aerial spores. Mycelial seg- ments to by to m- Gram-posi- tive; not acid-fast. Glucose-asparagine agar: Growth limited. Colonies mm, white, yellow reverse. Aerial mycelium snow-white. Soluble pig- ment none. Calcium malate agar: Growth limited. Nutrient agar: Growth limited. Colonies whitish-yellow, mm. Xo aerial mycelium. Starch agar: Growth limited. Aerial my- celium snow-white; reverse yellowish. Starch not hydrolyzed. Potato: Xo growth. Gelatin: Growth in form of microscopic colonies. Xo liquefaction. Xo pigmentation. Milk: Growth limited, yellowish, dry. Xo aerial mycelium. Xo coagulation; no pep- tonization. Temperature: Optimum 25-28°C. Re- sistant to drying. Salt concentration: Resistant to high concentration. Habitat: Soil. 42. Nocardia polychromogenes (Vallee, 1903) Waksman and Henrici, 1948 (Vallee, H. Ann. inst. Pasteur 17: 288-292, 1903; Jensen, II. Proc Linnean Soc. Wales 56: 79, 363, 1931). Morphology: Growth bright red, coral- red to red-pink, of a doughy consistency, later becoming leathery. Aerial mycelium wh
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