. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 6 FIGURE 5. Autoradiograph prepared from a whole-mount of destalked tiddler crab epi- demis with both dispersed and contracted pigment; upper pigment mass contracted, bar -- 20 p. FIGURE 6. Autoradiograph prepared from a whole-mount of destalked fiddler crab epi- dermis. Contracted pigment mass ruptured releasing pigment granules. Lower density of granules enables distinguishing silver grains from melanin granules, bar = 20 /*. Figure 4 is high-power phase optical view of a melanophore process. This section was analyzed in a
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 6 FIGURE 5. Autoradiograph prepared from a whole-mount of destalked tiddler crab epi- demis with both dispersed and contracted pigment; upper pigment mass contracted, bar -- 20 p. FIGURE 6. Autoradiograph prepared from a whole-mount of destalked fiddler crab epi- dermis. Contracted pigment mass ruptured releasing pigment granules. Lower density of granules enables distinguishing silver grains from melanin granules, bar = 20 /*. Figure 4 is high-power phase optical view of a melanophore process. This section was analyzed in a fashion similar to Figure 1. Twenty squares not con- taining any portion of a melanophore had ± silver grains per square as compared with ± grains per square over a portion of the melanophore. These grain counts are significantly different (P < ). Crabs whose eye-stalks have been removed have melanin concentrated in the center of the melanophore (Fig. 5). In this condition it is difficult to distinguish silver grains from melanin granules because of the high density of the latter. Only at the edges of the mass of melanin can silver grains be distinguished. In a number of cases preparative treatment of the tissue led to rupture of the concentrated mass of melanin. In these fortuitous cases the density of the melanin granules is reduced and the silver grains can be distinguished ( Fig. 6). In thick sections prepared as for electron microscopy it can be seen that the melanophores are present on both the environmental and hemocoel surfaces of the epidermis (Fig. 7ab). Figure 8 shows a comparable section with the pigment partially dispersed. Tissue with dispersed pigment is considerably more resistant. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Marine Biological Laboratory (Woods
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
