. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. Figure 5. Double-label, immunofluorescence micrograph of an in- testinal epithelial cell of Anguilla japonica incorporating fixed Vihrin anguillarum in vivo. Fixed Vibrio anguillarum ( mg) were injected into the lumen of the intestine. Thirty minutes later, intestinal cells were separated, fixed, and treated with methanol. After binding with 8F1 an- tibody, the cells were labeled with rhodamine-conjugated goat anti-mouse Ig(G + M) and rabbit anti-I'/Vir/o anguillarum antisera and finally with FITC-conjugated goat anti-rab
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. Figure 5. Double-label, immunofluorescence micrograph of an in- testinal epithelial cell of Anguilla japonica incorporating fixed Vihrin anguillarum in vivo. Fixed Vibrio anguillarum ( mg) were injected into the lumen of the intestine. Thirty minutes later, intestinal cells were separated, fixed, and treated with methanol. After binding with 8F1 an- tibody, the cells were labeled with rhodamine-conjugated goat anti-mouse Ig(G + M) and rabbit anti-I'/Vir/o anguillarum antisera and finally with FITC-conjugated goat anti-rabbit IgG. (A) A Vibrio anguillarum-iacor- porated cell visualized with anti-Vibrio anguillarum antiserum and the FITC-conjugated secondary antibody; (B) the same cell harboring the 8Fl-antigen proteins, identified by their labeling with the rhodamine- conjugated antibody. Bar, 5 ^m. macromolecules, such as horseradish peroxidase and fer- ritin (Bockman and Cooper, 1973; Owen. 1977). Fur- thermore, viruses (such as reovirus) were phagocytosed into the cells (Wolf el ai. 1981; Morrison el a/.. 1991). Such an endocytic process and the subsequent release of the ingested material into the intercellular space is con-. Figure 6. Immunofluorescence micrograph of a section of an intes- tinal segment of Anguilla japonica which was stained with 8FI and FITC- conjugated rabbit anti-mouse IgG. (A). A section observed by fluorescence microscopy; (B) the same field observed under bnghtfield microscopy. Bar, 10 Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Marine Biological Laboratory (Woods Hole, Mass. ); Marine Biological Laboratory (Woods Hole, Mass. ). Annual report 1907/08-1952; Lillie, Frank Rattray, 1870-1947; Moore, Carl Richard, 1892-; Redfield, Alfred Clarence, 1890-1983. Woods Hole, Mass. : Marine Biologica
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