. A laboratory guide in bacteriology, for the use of students, teachers, and practitioners. Litmus milk. Dimhams solution, if a test for indol is to transfers, excepting gelatin, are to be placedin the thermostat, unless otherwise ordered. 4. An accurate description and sketches are to bemade of each culture (see Section 9). These descrip-tions should be made complete after 24 hours, and anychanges should be noted after 48 hours and after 6days. (See culture charts.) 5. Plates are to be made in agar from a 24-48-hour-old broth culture of each culture, unless otherwised
. A laboratory guide in bacteriology, for the use of students, teachers, and practitioners. Litmus milk. Dimhams solution, if a test for indol is to transfers, excepting gelatin, are to be placedin the thermostat, unless otherwise ordered. 4. An accurate description and sketches are to bemade of each culture (see Section 9). These descrip-tions should be made complete after 24 hours, and anychanges should be noted after 48 hours and after 6days. (See culture charts.) 5. Plates are to be made in agar from a 24-48-hour-old broth culture of each culture, unless otherwisedirected. These plates are to be described once after24-48 hours. (See Section 9.) Note.—^The thermostat or incubator (Fig. 20) is a boxmade of copper and having double walls, between which water 52 LABORATORY GUIDE IN BACTERIOLOGY circulates. The outer surface is usually covered with asbestosor linoleiun, so as to hold the heat. The thermostat is providedwith two doors, the inner one of glass so as to enable the observerto look inside without causing a drop in temperature by admitting. Fig. 20Theimostat or Incubator the cooler air. The outer door is covered with asbestos likethe walls. A thermometer reaches through the upper part,provided with several air holes which permit free circulationof air. The gas supply is shut ofi automatically, if it shouldaccidentally become extinguished. The necessary heat may also BACTERIOLOGICAL TECHNIC S3 be obtained successfully by electric devices. For class use largeincubators are constructed on similar principles, with a numberof separate compartments for the accommodation of large num-bers of students. SECTION 9 METHOD OF DESCRIBING CULTURES The following method of describing cultures shouldbe carefully studied, and each suggestion should beconsidered in the description. It is of prime impor-tance that cultures should be closely observed, in allmedia, and accurately described and sketched, as thisis the only method which furnishes, the pr
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