. The Biological bulletin. Biology; Zoology; Marine biology. ATTACHMENT OF CELLS FROM PRASIOLA 267 PENIKESE " MANOMET (JULY, 1973) 10 -* _^MANOMET 9-TT^X* (JUNE, 1973) 2 6 10 14 18 22 26 30 TIME (HOURS) Figure 9. Variation of attachment kinetics with place of collection and time of collection of the Prasiola plants. The collection at Manomet in July, 1973 was the only one done in the rain. All cells were diluted to 5 X 10r' cells/ml for the attachment assay. relieved by addition of sulfate. Thus there is strong reason to believe that attachment requires the formation of a sulfated
. The Biological bulletin. Biology; Zoology; Marine biology. ATTACHMENT OF CELLS FROM PRASIOLA 267 PENIKESE " MANOMET (JULY, 1973) 10 -* _^MANOMET 9-TT^X* (JUNE, 1973) 2 6 10 14 18 22 26 30 TIME (HOURS) Figure 9. Variation of attachment kinetics with place of collection and time of collection of the Prasiola plants. The collection at Manomet in July, 1973 was the only one done in the rain. All cells were diluted to 5 X 10r' cells/ml for the attachment assay. relieved by addition of sulfate. Thus there is strong reason to believe that attachment requires the formation of a sulfated polysaccharide. Figure 8 shows the influence of molybdate on cells which have already attached to the substrate. Addition of molybdate alone causes cells to disattach from the substrate. The presence of sulfate partially reverses the molybdate effect. In the absence of molybdate or sulfate the cells remain attached. The fact that attachment is reversed by molybdate indicates possible turnover in the sulfated polysaccharide thought to be necessary for attachment. The attachment curves of cells released from Prasiola stipitata by grinding were not identical for material obtained from various locations at various times. Fig- ure 9 shows attachment of single cells under constant laboratory conditions where the cells were obtained from various collections of plants. While somewhat variable, most of the collections showed good attachment within ten to twenty hrs. An exception was the material collected from Manomet in July of 1973 which attached much more slowly than the material from other collections; collection in another year at the same time showed more rapid attachment. The only variable which seems to be different is that the slow material was the only collection to be made in the rain. Since distilled water will not support attachment (see above) perhaps there was sufficient rainwater in this material to inhibit the attachment process somewhat. Discussion It is possible to a
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