. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. PHYSIOLOGICAL EFFECTS OF HYDROGEN CYANIDE. 367 both the " cell " and bottle fitted with rubber stoppers. The in- side of the " cell " was filled with a borax-boric acid buffer solu- tion and a solution of HCN in a borax buffer was placed in the quinine bottle. It was necessary to use a buffer which would not injure the skin or react chemically with either the cyanide «or the silver nitrate solution used to determine the concentration of cya- nide. The pH values from to were obtained by changing H-
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. PHYSIOLOGICAL EFFECTS OF HYDROGEN CYANIDE. 367 both the " cell " and bottle fitted with rubber stoppers. The in- side of the " cell " was filled with a borax-boric acid buffer solu- tion and a solution of HCN in a borax buffer was placed in the quinine bottle. It was necessary to use a buffer which would not injure the skin or react chemically with either the cyanide «or the silver nitrate solution used to determine the concentration of cya- nide. The pH values from to were obtained by changing H-0 LJ °30 LJ CL 20 0. A 10 20 30. H-0 50 MINUTES 60 FIG. i. Curve showing the relation of the position of the skin to the permeability of the skin to cyanide. A, skin with the flesh side out; B, skin normal, flesh side in. Abscissae, time in minutes; ordinates per cent, cyanide. the relative amounts of sodium borate and boric acid; for pH values below it was necessary to add small amounts of nitric acid. The addition of HCN did not change the pH of the solu- tion of either the borax or the borax plus nitric acid. Pure liquid hydrogen cyanide was used. No leakage occurred around the skin as shown by the fact that when a " cell " was filled with an. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Marine Biological Laboratory (Woods Hole, Mass. ); Marine Biological Laboratory (Woods Hole, Mass. ). Annual report 1907/08-1952; Lillie, Frank Rattray, 1870-1947; Moore, Carl Richard, 1892-; Redfield, Alfred Clarence, 1890-1983. Woods Hole, Mass. : Marine Biological Laboratory
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