. Colchicine in agriculture, medicine, biology, and chemistry. Colchicine; Colchicine. '% B t^. Ci D Fig. —Pollen tube cultures treated and untreated. A. A metaphase of generative cell of Lllium michiganensis without treatment. One per cent agar and 7 per cent sucrose, stained with iron alum haemotoxylin. B. Anaphase, Polygonatum commutatum un- treated. Stained with acetocarmine. C. Two microgametes and tube nucleus. D. Ar- rested metaphase, c-pairs, caused by adding per cent colchicine to culture media. The duplications among c-pairs indicate polyploidy. There are 20 c-pairs but only


. Colchicine in agriculture, medicine, biology, and chemistry. Colchicine; Colchicine. '% B t^. Ci D Fig. —Pollen tube cultures treated and untreated. A. A metaphase of generative cell of Lllium michiganensis without treatment. One per cent agar and 7 per cent sucrose, stained with iron alum haemotoxylin. B. Anaphase, Polygonatum commutatum un- treated. Stained with acetocarmine. C. Two microgametes and tube nucleus. D. Ar- rested metaphase, c-pairs, caused by adding per cent colchicine to culture media. The duplications among c-pairs indicate polyploidy. There are 20 c-pairs but only 10 types for the entire group. Centromeric locus shown by incision along chromosomes. Stained with acetocarmine. (Eigsti, 1940). Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Eigsti, O. J. (Orie Jacob), 1908-; Dustin, Pierre. Ames, Iowa State College Press


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