. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 260 REPORTS FROM THE MBL GENERAL SCIENTIFIC MEETINGS steps of 10 and 100 n\l hydroxyproline (Fig. 1A). The receptor cells were allowed to recover for at least 90 s between each stim- ulus series. Cell responses were quantified by the total number of spikes and by the maximum number of spikes in any 100- ms time period within a response. Fifteen receptor cells survived the entire stimulus protocol and were used for data analysis. Cells responded to 10 and 100 nM test pulses with brief bursts of ± and ± spike
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 260 REPORTS FROM THE MBL GENERAL SCIENTIFIC MEETINGS steps of 10 and 100 n\l hydroxyproline (Fig. 1A). The receptor cells were allowed to recover for at least 90 s between each stim- ulus series. Cell responses were quantified by the total number of spikes and by the maximum number of spikes in any 100- ms time period within a response. Fifteen receptor cells survived the entire stimulus protocol and were used for data analysis. Cells responded to 10 and 100 nM test pulses with brief bursts of ± and ± spikes, respectively. Responses to the adapting pulse were phasic and terminated even when the odor was still present, indicating that the receptor cells completely adapted to the stim- ulus. After a 1 s recovery time, only 30% of the receptor cells responded to the probe pulse. When given a longer recovery time, individual receptor cells regained their sensitivity at dif- ferent rates. After 30 s, all receptor cells recovered 90% of their original response: the magnitude of the probe pulse responses was not significantly different from the test pulse response mag- nitude (P < , paired Mest) for both stimulus concentrations used. To assess the time course of recovery of the cell population, we compared the mean response values of the 15 cells for test and probe pulses (Fig. IB). Responses fully recovered within 25 to 30 s; beyond 30 s. responses did not significantly increase. Surprisingly, the time course of recovery was the same for both stimulus concentrations. Although full recovery took 30 s, cells regained 50% of their sensitivity within 9 s. Hydroxyproline-sensitive cells can follow 2 Hz pulse trains (3). It is the time course of recovery from adaptation that integrates odor events and sets the high frequency limit of the system for detection of the spectrum of odor con- centration fluctuations that occurs in an odor plume. This work was supported by NSF IBN 9222774
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
