. A laboratory guide in bacteriology, for the use of students, teachers and practitioners. Bacteriology. BACTERIOLOGICAL TECHNIC 11 certain chemicals, or by filtration. Chemicals are used chiefly for sterilizing the skin, surgical instru- ments, and cultures which have been accidentally spilt. Filters for sterilization are made of some porous material, either infusorial earth or unglazed porcelain. Substances which may be injured by heat are sterilized in this manner. Positive or negative pressure is necessary for this kind of sterilization (Fig. 3). Sterilization by dry heat. —Sterilization b
. A laboratory guide in bacteriology, for the use of students, teachers and practitioners. Bacteriology. BACTERIOLOGICAL TECHNIC 11 certain chemicals, or by filtration. Chemicals are used chiefly for sterilizing the skin, surgical instru- ments, and cultures which have been accidentally spilt. Filters for sterilization are made of some porous material, either infusorial earth or unglazed porcelain. Substances which may be injured by heat are sterilized in this manner. Positive or negative pressure is necessary for this kind of sterilization (Fig. 3). Sterilization by dry heat. —Sterilization by dry heat is appUcable to the sterihza- tion of most glassware. This method of sterilization is car- ried out by means of hot-air sterilizers (Figs. 4 and 5). fig. 4 T-iT. Li- i •! • Koch's Hot-Air Sterilizer ihese hot-air sterilizers are boxes with double walls of sheet iron. The bottom shelf should always be covered with a piece of asbes- tos, to prevent lieating the apparatus too rapidly. The temperature is maintained at 160° or more for one hour. The flame enters a hole provided at the bottom of the box. Care should be taken to avoid the possi- biUty of the flame becoming luminous, otherwise the glassware will be covered with soot. Culture media and all substances liable to be in- jured by heat of 160° C. or over must be sterilized by the application of moist heat. Experience has taught that hot steam has greater germicidal powers than air of the same temperature. Hot steam, therefore, is the most common means of sterilizing culture Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Heinemann, Paul Gustav. Chicago, Ill. , The University of Chicago Press
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