. Calcified tissues; proceedings. Bone; Collagen; Calcification. 46 A. B. BORLE bone, kidney and intestine (Borle ct ai, 1960; Egawa and Neuman, 1963; Borle et ai, 1963). In HeLa cells, a) PTH increased lactate production ZS-S^/o from ± to ± « cells (p< ); b) PTH increased the cellular -^^•^ '^:. r^: Pi 21''/o; c) PTH increased ^-P incorporation into the lipid fraction of the cells 63Vo in one hour (relative specific activity ;^ in control vs. ;^ in the experimental group). In monkey kidney cells, PTH did not increase lactate produc-
. Calcified tissues; proceedings. Bone; Collagen; Calcification. 46 A. B. BORLE bone, kidney and intestine (Borle ct ai, 1960; Egawa and Neuman, 1963; Borle et ai, 1963). In HeLa cells, a) PTH increased lactate production ZS-S^/o from ± to ± « cells (p< ); b) PTH increased the cellular -^^•^ '^:. r^: Pi 21''/o; c) PTH increased ^-P incorporation into the lipid fraction of the cells 63Vo in one hour (relative specific activity ;^ in control vs. ;^ in the experimental group). In monkey kidney cells, PTH did not increase lactate produc- tion but increased the total cellular phosphate 30^/0 from to P/mg cell protein (p<C ). Table 1 presents a summary of these changes and a comparison Table 1. Metabolic effects of PTH Lactate Coll P Production I'ptake 3 21. lipid HeLa Cells . . Monkey kidney cells Bone slices 1' - . . Kidney slices - . . Intestine-^ (LE Ct al., 1960. + 30% I (+ 21%) 0 I + 30% + 34% I + 38% - I + 26% + 36%, I + 28% Egawa and Neuman, 1963. + 63°o + 34% » Borle et al, 1963. with the results previously obtained in bone, kidney and intestine. It appears from these studies, that PTH may enhance aerobic glycolysis, phosphate transport into the cell and probably the turnover of phospholipids. In view of the morphological observations reported above, it is of interest that very similar metabolic changes have been reported in phagocytosing leucocytes (Sbarra and Karnovsky, 1959, 1960).. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original European Symposium on Calcified Tissues; European Bone and Tooth Symposium. Berlin, New York [etc. ] Springer-Verlag [etc. ]
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