. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. B MALES. Figure 3. Frequencies of hyaline (H), semigranular(SG). and granular (G) hemocyte cell populations found in Pemieus japonicu\ hemolymph at the indicated stages of the molting cycle in females (A) and males (B). Analyses have been performed with 10,000 cells from each animal; n indicates the number of animals used for each value, which is presented as the mean and 1 standard deviation. Cells of one of these populations were classified as G cells because their peroxidase activity was like that previously observed in s
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. B MALES. Figure 3. Frequencies of hyaline (H), semigranular(SG). and granular (G) hemocyte cell populations found in Pemieus japonicu\ hemolymph at the indicated stages of the molting cycle in females (A) and males (B). Analyses have been performed with 10,000 cells from each animal; n indicates the number of animals used for each value, which is presented as the mean and 1 standard deviation. Cells of one of these populations were classified as G cells because their peroxidase activity was like that previously observed in similar cells in Procambarus clarki (Lanz et 1993). When examined by light microscopy, the G cells appear slightly larger than the SG cells, but our flow cytometric results indicate that this may not be the case. This discrepancy might result from analyzing fresh cell suspensions instead of the fixed hemocyte smears used in light microscopy. This study provides some evidence that the hemocyte cell populations of P. iaponicux exhibit sex-related vari- ations associated with the molt cycle. In both sexes, the relative percentages of H, SG, and G cells are identical after (stage B) and before (stage Dl) ecdysis. Similar results were reported by Bauchau and Plaquet (1973). However, that study, which relied on morphological observations of hemocyte smears, was not directly comparable because SG and G cells could not be identified and were thus counted together, and because the samples were not iden- tified by sex. More recently, Tsing et al. (1989) reported that no significant changes of the hemocyte cell popula- tions occurred in P. japonicus during the molting stages. However, this study, like the one by Bauchau and Plaquet (1973), pooled male and female samples and was based on smear observations; furthermore, more than 50% of the hemocytes could not be identified. It seems, then, that flow cytometric analysis is a better and more reliable method for studying hemocyte variati
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