. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 234 MARGARKT \. HARRISON1 and shaken vigorously for 15 seconds. \Yhen it liad formed, ml. of the upper phase were transferred with a syringe into a centrifuge tube and made up to 5 ml. with acid alcohol. Stannous chloride reagent ( ml.) was then added, and after 20 minutes the optical density at 625 mp. was determined with the colorimeter mentioned above. A blank and two standards ( ) were run simultaneously. The method of Martin and Doty (1949) was also used for the determination of i.'tal phosphorus and tota
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 234 MARGARKT \. HARRISON1 and shaken vigorously for 15 seconds. \Yhen it liad formed, ml. of the upper phase were transferred with a syringe into a centrifuge tube and made up to 5 ml. with acid alcohol. Stannous chloride reagent ( ml.) was then added, and after 20 minutes the optical density at 625 mp. was determined with the colorimeter mentioned above. A blank and two standards ( ) were run simultaneously. The method of Martin and Doty (1949) was also used for the determination of i.'tal phosphorus and total acid-soluble phosphorus, but with the modifications sug- gested by Ernster ct al. (1950). Two test tubes were prepared, one (total. HOURS IN NITROGEN K 4. Changes in ereatine and phosphorus fractions in Kana pipicns embryos at Stage 11 after short exposure to anaerobiosis. phosphorus) containing ml. of homogenate, the other (total acid-soluble phos- phorus) containing 1 nil. of protein-free extract. After the addition of 1 ml. of 10 X 1I,S( ), to each tube, they were placed in an oven at 130-160° C. After two to three hours, and after partial cooling, one drop of 30^ was added to each tube, which was then heated over a bunsen burner until the contents became colorless or very pale yellow in color. The tubes were then replaced in the oven for 15 minutes. After partial cooling, 1 ml. of water was added to each tube, which was then returned to the oven, now at 100° C., for 10 minutes. The tubes were then cooled, and the contents made up to 7 ml. with water. Inorganic phosphorus present was then determined as described above, with the exception that H,SO, was omitted from the molybdate Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Marine Biological Laboratory (Woods Hole, Mass. ); Marine
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
