A text book of physiology . n separated from the other constituents of the serum, haemo-globin appears as a substance, either amorphous or crystalline,readily soluble in water (especially in warm water) and in serum. Since it is soluble in serum, and since the identity of the crystalsobserved occasionally within the corpuscles with those obtained in otherways shews that the haemoglobin as it exists in the corpuscle is the samethiug as that which is artificially prepared from blood, it is evident thatsome peculiar relationship between the stroma and the haemoglobin must,in natural blood, keep t
A text book of physiology . n separated from the other constituents of the serum, haemo-globin appears as a substance, either amorphous or crystalline,readily soluble in water (especially in warm water) and in serum. Since it is soluble in serum, and since the identity of the crystalsobserved occasionally within the corpuscles with those obtained in otherways shews that the haemoglobin as it exists in the corpuscle is the samethiug as that which is artificially prepared from blood, it is evident thatsome peculiar relationship between the stroma and the haemoglobin must,in natural blood, keep the latter from being dissolved by the in preparing haemoglobin it is necessaiy first of all to break up thecorpuscles. This may be done by the addition of chloroform or of bilesalts, or by repeatedly freezing and thawing. It is also of advantagepreviously to remove the alkaline serum, so as to operate only on the redcorpuscles. The corpuscles being thus broken up, an aqueous solution of 314 HEMOGLOBIN. [Book Chap, ii.] RESPIRATION-. 315 Fig. 49. The Spectra op Hemoglobin and some of its derivatives shewn in EEFERENCE TO FeAUENHOFERs LINES. The first spectrum of oxyhemoglobin is that of an exceedingly dilute solution. Thatof a solution intermediate between the first and second spectra would resemble inthe intensity of its absorption-bands the spectrum given as that of carbonic oxidehaemoglobin. haemoglobin is the result. The alkalinity of the solution, when present,being reduced by the cautious addition of dilute acetic acid, and the solventpower of the aqueous medium being diminished by the addition of onefourth its bulk of alcohol, the mixture, set aside in a temperature of 0° further to reduce the solubility of the haemoglobin, readily crystallizes,when the blood used is that of the dog, cat, horse, rat, guinea-pig, & crystals may be separated by filtration, redissolved in water and re-crystallized. Haemoglobin from the blood of the rat, guinea-
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